Application | Comment | Organism |
---|---|---|
agriculture | in mature poplar leaves, isoprene emission is the main metabolic sink of plastidic isoprenoid intermediates. Consequently, there is lower enzymic activity in non-isoprene-emitting lines of poplar than in emitting plants as indicator of a lower demand of metabolic flux within the MEP pathway | Populus tremula x Populus alba |
analysis | development of a sensitive assay suitable for plant extracts that is based on the decarboxylation of labeled pyruvate and detection of 13CO2 by isotope ratio mass spectrometry | Populus tremula x Populus alba |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-fluoro-2-oxopropanoate | competitive, 0.1 mM, up to 57% inhibition | Populus tremula x Populus alba |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.111 | - |
pyruvate | pH 8.5, 40°C | Populus tremula x Populus alba | |
0.158 | - |
D-glyceraldehyde 3-phosphate | pH 8.5, 40°C | Populus tremula x Populus alba |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Populus tremula x Populus alba | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | activity strongly depended on leaf development with higher activity in young leaves and correlated fairly well with leaf isoprene emission potential | Populus tremula x Populus alba | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate + D-glyceraldehyde 3-phosphate | - |
Populus tremula x Populus alba | 1-deoxy-D-xylulose 5-phosphate + CO2 | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Populus tremula x Populus alba |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.6 | - |
- |
Populus tremula x Populus alba |