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Literature summary for 2.2.1.7 extracted from
- The single cellular green microalga Botryococcus braunii, race B possesses three distinct 1-deoxy-D-xylulose 5-phosphate synthases (2012), Plant Sci., 185-186, 309-320.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Botryococcus braunii |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.47 | - |
D-glyceraldehyde 3-phosphate | pH 7.8, 32°C | Botryococcus braunii |  |
| 0.72 | - |
pyruvate | pH 7.8, 32°C | Botryococcus braunii | |
| 0.75 | - |
pyruvate | pH 7.8, 32°C | Botryococcus braunii | |
| 0.87 | - |
D-glyceraldehyde 3-phosphate | pH 7.8, 32°C | Botryococcus braunii | |
| 1 | - |
D-glyceraldehyde 3-phosphate | pH 7.8, 32°C | Botryococcus braunii | |
| 1.8 | - |
pyruvate | pH 7.8, 32°C | Botryococcus braunii | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| plastid | sequence contains a plastid targeting peptide | Botryococcus braunii | 9536 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required, maximal activity at 4-12 mM | Botryococcus braunii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 72500 | - |
x * 78500, calculated including plastid targeting peptide, x * 72500, calculated for mature protein | Botryococcus braunii |
| 77400 | - |
x * 82300, calculated including plastid targeting peptide, x * 77400, calculated for mature protein | Botryococcus braunii |
| 77400 | - |
x * 83100, calculated including plastid targeting peptide, x * 77400, calculated for mature protein | Botryococcus braunii |
| 78500 | - |
x * 78500, calculated including plastid targeting peptide, x * 72500, calculated for mature protein | Botryococcus braunii |
| 82300 | - |
x * 82300, calculated including plastid targeting peptide, x * 77400, calculated for mature protein | Botryococcus braunii |
| 83100 | - |
x * 83100, calculated including plastid targeting peptide, x * 77400, calculated for mature protein | Botryococcus braunii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Botryococcus braunii | H5ZXI0 | isoform DXS-II | - |
| Botryococcus braunii | H5ZXI1 | isoform DXS-II | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| proteolytic modification | sequence contains a plastid targeting peptide | Botryococcus braunii |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, elution buffer containing 30% glycerol, storage for around 10 days with no significant loss of activity | Botryococcus braunii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pyruvate + D-glyceraldehyde 3-phosphate | - |
Botryococcus braunii | 1-deoxy-D-xylulose 5-phosphate + CO2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 78500, calculated including plastid targeting peptide, x * 72500, calculated for mature protein | Botryococcus braunii |
| ? | x * 82300, calculated including plastid targeting peptide, x * 77400, calculated for mature protein | Botryococcus braunii |
| ? | x * 83100, calculated including plastid targeting peptide, x * 77400, calculated for mature protein | Botryococcus braunii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DXS-I | - |
Botryococcus braunii |
| DXS-II | - |
Botryococcus braunii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 32 | - |
- |
Botryococcus braunii |
| 34 | - |
- |
Botryococcus braunii |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
75% of the activity at 34°C | Botryococcus braunii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
sharp decrease in activity above | Botryococcus braunii |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.9 | - |
pyruvate | pH 7.8, 32°C | Botryococcus braunii | |
| 2 | - |
D-glyceraldehyde 3-phosphate | pH 7.8, 32°C | Botryococcus braunii | |
| 2.4 | - |
pyruvate | pH 7.8, 32°C | Botryococcus braunii | |
| 5.6 | - |
pyruvate | pH 7.8, 32°C | Botryococcus braunii | |
| 6 | - |
D-glyceraldehyde 3-phosphate | pH 7.8, 32°C | Botryococcus braunii | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | 9 | - |
Botryococcus braunii |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Botryococcus braunii | mRNA accumulation patterns over time are almost identical among the three DXS isoforms reaching the highest expression levels on day 6 after inoculation into fresh medium | additional information |
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