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Literature summary for 2.2.1.7 extracted from

  • Banerjee, A.; Wu, Y.; Banerjee, R.; Li, Y.; Yan, H.; Sharkey, T.D.
    Feedback inhibition of deoxy-D-xylulose-5-phosphate synthase regulates the methylerythritol 4-phosphate pathway (2013), J. Biol. Chem., 288, 16926-16936.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)pLysS cells Populus trichocarpa

Inhibitors

Inhibitors Comment Organism Structure
dimethylallyl diphosphate competitive inhibitor Populus trichocarpa
isopentenyl diphosphate competitive inhibitor Populus trichocarpa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0185
-
D-glyceraldehyde 3-phosphate at pH 8.0 and 37°C Populus trichocarpa
0.0878
-
pyruvate at pH 8.0 and 37°C Populus trichocarpa

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
Populus trichocarpa 9507
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Populus trichocarpa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pyruvate + D-glyceraldehyde 3-phosphate Populus trichocarpa
-
1-deoxy-D-xylulose 5-phosphate + CO2
-
?

Organism

Organism UniProt Comment Textmining
Populus trichocarpa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation and Ni-NTA column chromatography Populus trichocarpa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyruvate + D-glyceraldehyde 3-phosphate
-
Populus trichocarpa 1-deoxy-D-xylulose 5-phosphate + CO2
-
?

Synonyms

Synonyms Comment Organism
deoxyxylulose-5-phosphate synthase
-
Populus trichocarpa
DXS
-
Populus trichocarpa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.53
-
pyruvate at pH 8.0 and 37°C Populus trichocarpa
0.64
-
D-glyceraldehyde 3-phosphate at pH 8.0 and 37°C Populus trichocarpa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Populus trichocarpa

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8.5 the enzyme does not show substantial activity below pH 6.5 and above pH 8.5 Populus trichocarpa

Cofactor

Cofactor Comment Organism Structure
thiamine diphosphate
-
Populus trichocarpa

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0654
-
isopentenyl diphosphate at pH 8.0 and 37°C Populus trichocarpa
0.0813
-
dimethylallyl diphosphate at pH 8.0 and 37°C Populus trichocarpa