Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)pLysS cells | Populus trichocarpa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dimethylallyl diphosphate | competitive inhibitor | Populus trichocarpa | |
isopentenyl diphosphate | competitive inhibitor | Populus trichocarpa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0185 | - |
D-glyceraldehyde 3-phosphate | at pH 8.0 and 37°C | Populus trichocarpa | |
0.0878 | - |
pyruvate | at pH 8.0 and 37°C | Populus trichocarpa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Populus trichocarpa | 9507 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Populus trichocarpa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate + D-glyceraldehyde 3-phosphate | Populus trichocarpa | - |
1-deoxy-D-xylulose 5-phosphate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Populus trichocarpa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation and Ni-NTA column chromatography | Populus trichocarpa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate + D-glyceraldehyde 3-phosphate | - |
Populus trichocarpa | 1-deoxy-D-xylulose 5-phosphate + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
deoxyxylulose-5-phosphate synthase | - |
Populus trichocarpa |
DXS | - |
Populus trichocarpa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.53 | - |
pyruvate | at pH 8.0 and 37°C | Populus trichocarpa | |
0.64 | - |
D-glyceraldehyde 3-phosphate | at pH 8.0 and 37°C | Populus trichocarpa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Populus trichocarpa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8.5 | the enzyme does not show substantial activity below pH 6.5 and above pH 8.5 | Populus trichocarpa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | - |
Populus trichocarpa |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0654 | - |
isopentenyl diphosphate | at pH 8.0 and 37°C | Populus trichocarpa | |
0.0813 | - |
dimethylallyl diphosphate | at pH 8.0 and 37°C | Populus trichocarpa |