General Stability | Organism |
---|---|
glycerol stabilizes | Escherichia coli |
L-arginine or N-acetyl-L-glutamate stabilizes the high molecular weight form of 300000 Da | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
arginine | 0.02 mM, 50% inhibition; L-arginine | Escherichia coli | |
Cd2+ | 0.1 mM, 94% inhibition | Escherichia coli | |
coenzyme A | 2.5 mM, 50% inhibition | Escherichia coli | |
Cu2+ | 0.1 mM, 68% inhibition | Escherichia coli | |
Hg2+ | 0.1 mM; 50% inhibition | Escherichia coli | |
high ionic strength | - |
Escherichia coli | |
KCl | 100 mM, 36% inhibition after 1 h, 300 mM, 74% inhibition after 1 h, 500 mM, 87% inhibition after 1 h | Escherichia coli | |
L-citrulline | 10 mM, 75% inhibition | Escherichia coli | |
N-acetylglutamate | 25 mM, 50% inhibition | Escherichia coli | |
N-ethylmaleimide | - |
Escherichia coli | |
p-chloromercuribenzoate | - |
Escherichia coli | |
p-hydroxymercuribenzoate | - |
Escherichia coli | |
Zn2+ | 0.1 mM, 43% inhibition | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51700 | - |
6 * 51700, SDS-PAGE, cross-linking studies with dimethylsuberimidate, in the maximally aggregated state the enzyme exists as a hexamer | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-cellulose, hydroxylapatite | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
133 | - |
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + L-glutamate | - |
Escherichia coli | CoA + N-acetyl-L-glutamate | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 51700, SDS-PAGE, cross-linking studies with dimethylsuberimidate, in the maximally aggregated state the enzyme exists as a hexamer | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10 | - |
- |
Escherichia coli |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | - |
highest stability | Escherichia coli |