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Literature summary for 2.3.1.1 extracted from

  • Haskins, N.; Panglao, M.; Qu, Q.; Majumdar, H.; Cabrera-Luque, J.; Morizono, H.; Tuchman, M.; Caldovic, L.
    Inversion of allosteric effect of arginine on N-acetylglutamate synthase, a molecular marker for evolution of tetrapods (2008), BMC Biochem., 9, 24.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.1

Activating Compound

Activating Compound Comment Organism Structure
arginine
-
 Mus musculus
arginine
-
 Xenopus laevis
arginine
-
 Xenopus tropicalis

Application

Application Comment Organism
additional information N-acetylglutamate synthase is a molecular marker for evolution of tetrapods Pseudomonas aeruginosa

Cloned(Commentary)

Cloned (Comment) Organism
cloned in Escherichia coli as a His-tagged fusion protein Mus musculus
cloned in Escherichia coli as a His-tagged fusion protein Escherichia coli
cloned in Escherichia coli as a His-tagged fusion protein Arabidopsis thaliana
cloned in Escherichia coli as a His-tagged fusion protein Xenopus laevis
cloned in Escherichia coli as a His-tagged fusion protein Xanthomonas campestris
cloned in Escherichia coli as a His-tagged fusion protein Danio rerio
cloned in Escherichia coli as a His-tagged fusion protein Takifugu rubripes
cloned in Escherichia coli as a His-tagged fusion protein Xenopus tropicalis
cloned in Escherichia coli as a His-tagged fusion protein Maricaulis maris
phylogenetic analysis Pseudomonas aeruginosa

Protein Variants

Protein Variants Comment Organism
E280A specific activity (micromol/min/mg): 26.93, 26.68 (+ 1mM arginine), arginine inhibition is abolished Xanthomonas campestris
E280A specific activity (micromol/min/mg): 26.93, 26.68 (+1mM arginine), arginine inhibition is abolished Xanthomonas campestris
E354A specific activity (micromol/min/mg): 15.47, 15.28 (+ 1mM arginine), arginine activation is abolished Mus musculus
E354A specific activity (micromol/min/mg): 15.47, 15.28 (+1mM arginine), arginine activation is abolished Mus musculus
F121C specific activity (micromol/min/mg): 12.22, 13.08 (+ 1mM arginine), arginine activation is abolished Mus musculus
F121C specific activity (micromol/min/mg): 12.22, 13.08 (+1mM arginine), arginine activation is abolished Mus musculus
F35C specific activity (micromol/min/mg): 9.66, 4.31 (+ 1mM arginine), only partial inhibition by arginine Xanthomonas campestris
F35C specific activity (micromol/min/mg): 9.66, 4.31 (+1mM arginine), only partial inhibition by arginine Xanthomonas campestris
G286P specific activity (micromol/min/mg): 3.51, 3.39 (+ 1mM arginine), no inhibition by arginine Xanthomonas campestris
G286P specific activity (micromol/min/mg): 3.51, 3.39 (+1mM arginine), no inhibition by arginine Xanthomonas campestris
G288S specific activity (micromol/min/mg): 46.04, 42.55 (+ 1mM arginine), no inhibition by arginine Xanthomonas campestris
G288S specific activity (micromol/min/mg): 46.04, 42.55 (+1mM arginine), no inhibition by arginine Xanthomonas campestris
G360P specific activity (micromol/min/mg): 13.00, 12.57 (+ 1mM arginine), arginine activation is abolished Mus musculus
G360P specific activity (micromol/min/mg): 13.00, 12.57 (+1mM arginine), arginine activation is abolished Mus musculus
G362S specific activity (micromol/min/mg): 3.22, 3.19 (+ 1mM arginine), arginine activation is abolished Mus musculus
G362S specific activity (micromol/min/mg): 3.22, 3.19 (+1mM arginine), arginine activation is abolished Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
arginine
-
 Arabidopsis thaliana
arginine
-
 Danio rerio
arginine
-
 Escherichia coli
arginine
-
 Maricaulis maris
arginine the arginine binding site is localized in the kinase domain. Arginine is an allosteric inhibitor of microbial NAGS as part of feedback regulation of arginine biosynthesis, arginine is also an inhibitor of plant NAGS, a partial inhibitor of fish NAGS, but an allosteric activator of mammalian NAGS Pseudomonas aeruginosa
arginine
-
 Takifugu rubripes
arginine
-
 Xanthomonas campestris

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + L-glutamate Pseudomonas aeruginosa NAGS is the first enzyme of arginine biosynthesis, microbial arginine biosynthesis pathway, overview. Arginine is an allosteric inhibitor of microbial NAGS, arginine is also an inhibitor of plant NAGS, a partial inhibitor of fish NAGS, but an allosteric activator of mammalian NAGS CoA + N-acetyl-L-glutamate
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-
Danio rerio
-
-
-
Escherichia coli
-
-
-
Maricaulis maris
-
-
-
Mus musculus
-
-
-
Pseudomonas aeruginosa P22567
-
-
Takifugu rubripes
-
-
-
Xanthomonas campestris
-
-
-
Xenopus laevis
-
-
-
Xenopus tropicalis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using Ni-NTA chromatography Mus musculus
using Ni-NTA chromatography Escherichia coli
using Ni-NTA chromatography Arabidopsis thaliana
using Ni-NTA chromatography Xenopus laevis
using Ni-NTA chromatography Xanthomonas campestris
using Ni-NTA chromatography Danio rerio
using Ni-NTA chromatography Takifugu rubripes
using Ni-NTA chromatography Xenopus tropicalis
using Ni-NTA chromatography Maricaulis maris

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 no activity detectable upon addition of 1 mM arginine Arabidopsis thaliana
additional information
-
 no activity detectable upon addition of 1 mM arginine Xanthomonas campestris
0.098
-
 wild-type Xenopus tropicalis
0.136
-
 wild-type + 1 mM arginine Xenopus tropicalis
0.438
-
 wild-type Xenopus laevis
0.528
-
 wild-type + 1 mM arginine Xenopus laevis
0.572
-
 wild-type + 1mM arginine Maricaulis maris
1.84
-
 wild-type + 1mM arginine Escherichia coli
3.19
-
 mutant G362S + 1 mM arginine Mus musculus
3.22
-
 mutant G362S Mus musculus
3.39
-
 mutant G286P + 1 mM arginine Xanthomonas campestris
3.39
-
 mutant G286P + 1mM arginine Xanthomonas campestris
3.51
-
 mutant G286P Xanthomonas campestris
4.31
-
 mutant F35C + 1 mM arginine Xanthomonas campestris
4.31
-
 mutant F35C + 1mM arginine Xanthomonas campestris
5.475
-
 wild-type + 1 mM arginine Danio rerio
5.85
-
 wild-type Escherichia coli
9.11
-
 wild-type Arabidopsis thaliana
9.66
-
 mutant F35C Xanthomonas campestris
10.64
-
 wild-type Danio rerio
12.22
-
 mutant F121C Mus musculus
12.27
-
 wild-type Mus musculus
12.57
-
 mutant G360P + 1 mM arginine Mus musculus
12.57
-
 mutant G360P + 1mM arginine Mus musculus
13
-
 mutant G360P Mus musculus
13.08
-
 mutant F121C + 1 mM arginine Mus musculus
15.28
-
 mutant E354A + 1 mM arginine Mus musculus
15.47
-
 mutant E354A Mus musculus
15.89
-
 wild-type + 1 mM arginine Takifugu rubripes
20.88
-
 wild-type Takifugu rubripes
25.56
-
 wild-type + 1 mM arginine Mus musculus
26.68
-
 mutant E280A + 1 mM arginine Xanthomonas campestris
26.68
-
 mutant E280A + 1mM arginine Xanthomonas campestris
26.93
-
 mutant E280A Xanthomonas campestris
42.55
-
 mutant G288S + 1 mM arginine Xanthomonas campestris
42.55
-
 mutant G288S + 1mM arginine Xanthomonas campestris
46.04
-
 mutant G288S Xanthomonas campestris
62
-
 wild-type Maricaulis maris
71
-
 wild-type Xanthomonas campestris
111.7
-
 wild-type Xanthomonas campestris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + L-glutamate
-
 Pseudomonas aeruginosa CoA + N-acetyl-L-glutamate
-
 ?
acetyl-CoA + L-glutamate NAGS is the first enzyme of arginine biosynthesis, microbial arginine biosynthesis pathway, overview. Arginine is an allosteric inhibitor of microbial NAGS, arginine is also an inhibitor of plant NAGS, a partial inhibitor of fish NAGS, but an allosteric activator of mammalian NAGS Pseudomonas aeruginosa CoA + N-acetyl-L-glutamate
-
 ?

Synonyms

Synonyms Comment Organism
N-acetylglutamate synthase
-
 Mus musculus
N-acetylglutamate synthase
-
 Escherichia coli
N-acetylglutamate synthase
-
 Arabidopsis thaliana
N-acetylglutamate synthase
-
 Xenopus laevis
N-acetylglutamate synthase
-
 Xanthomonas campestris
N-acetylglutamate synthase
-
 Danio rerio
N-acetylglutamate synthase
-
 Takifugu rubripes
N-acetylglutamate synthase
-
 Xenopus tropicalis
N-acetylglutamate synthase
-
 Maricaulis maris
N-acetylglutamate synthase
-
 Pseudomonas aeruginosa
NAGS
-
 Mus musculus
NAGS
-
 Escherichia coli
NAGS
-
 Arabidopsis thaliana
NAGS
-
 Xenopus laevis
NAGS
-
 Xanthomonas campestris
NAGS
-
 Danio rerio
NAGS
-
 Takifugu rubripes
NAGS
-
 Xenopus tropicalis
NAGS
-
 Maricaulis maris
NAGS
-
 Pseudomonas aeruginosa
XcNAGS
-
 Xanthomonas campestris

Cofactor

Cofactor Comment Organism Structure
acetyl-CoA
-
 Pseudomonas aeruginosa