Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21 | Pseudomonas aeruginosa |
Protein Variants | Comment | Organism |
---|---|---|
delE283delQ284 | decrease of enzyme activity, induced substrate inhibition by acetyl-CoA | Pseudomonas aeruginosa |
delQ284 | increase of enzyme activity, triggered acetyl-CoA inhibition | Pseudomonas aeruginosa |
ins284A | lower enzyme activity, decreased inhibition by arginine | Pseudomonas aeruginosa |
ins284Ains285A | lower enzyme acitivity, decreased inhibition by arginine | Pseudomonas aeruginosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | - |
Pseudomonas aeruginosa | |
arginine | - |
Pseudomonas aeruginosa | |
L-glutamate | - |
Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
acetyl-CoA | wild-type enzyme, in presence of 2 mM arginine | Pseudomonas aeruginosa | |
0.07 | - |
acetyl-CoA | mutant ins284Ains285A | Pseudomonas aeruginosa | |
0.09 | - |
acetyl-CoA | wild-type enzyme | Pseudomonas aeruginosa | |
0.1 | - |
acetyl-CoA | mutant ins284A | Pseudomonas aeruginosa | |
0.13 | - |
acetyl-CoA | mutant ins284Ains285A, in presence of 2 mM arginine | Pseudomonas aeruginosa | |
0.14 | - |
acetyl-CoA | mutant ins284A, in presence of 2 mM arginine | Pseudomonas aeruginosa | |
0.15 | - |
acetyl-CoA | mutant delQ284, in presence of 2 mM arginine | Pseudomonas aeruginosa | |
0.22 | - |
acetyl-CoA | mutant delE283delQ284, in presence of 2 mM arginine | Pseudomonas aeruginosa | |
0.24 | - |
acetyl-CoA | mutant delQ284 | Pseudomonas aeruginosa | |
0.26 | - |
acetyl-CoA | mutant delE283delQ284 | Pseudomonas aeruginosa | |
2.1 | - |
L-glutamate | mutant ins284A | Pseudomonas aeruginosa | |
2.2 | - |
L-glutamate | mutant ins284A, in presence of 2 mM arginine | Pseudomonas aeruginosa | |
2.6 | - |
L-glutamate | mutant ins284Ains285A | Pseudomonas aeruginosa | |
4.9 | - |
L-glutamate | mutant ins284Ains285A, in presence of 2 mM arginine | Pseudomonas aeruginosa | |
5.2 | - |
L-glutamate | wild-type enzyme | Pseudomonas aeruginosa | |
20 | - |
L-glutamate | mutant delQ284 | Pseudomonas aeruginosa | |
85 | - |
L-glutamate | wild-type enzyme, in presence of 2 mM arginine | Pseudomonas aeruginosa | |
100 | - |
L-glutamate | mutant delE283delQ284 | Pseudomonas aeruginosa | |
106 | - |
L-glutamate | mutant delQ284, in presence of 2 mM arginine | Pseudomonas aeruginosa | |
107 | - |
L-glutamate | mutant delE283delQ284, in presence of 2 mM arginine | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas aeruginosa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
24 | - |
mutant delE283delQ284 | Pseudomonas aeruginosa |
43.6 | - |
mutant ins284Ains285A | Pseudomonas aeruginosa |
48.8 | - |
mutant ins284A | Pseudomonas aeruginosa |
79.4 | - |
wild-type enzyme | Pseudomonas aeruginosa |
114.9 | - |
mutant delQ284 | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + L-glutamate | - |
Pseudomonas aeruginosa | CoA + N-acetyl-L-glutamate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
N-acetyl-L-glutamate synthase | - |
Pseudomonas aeruginosa |
NAGS | - |
Pseudomonas aeruginosa |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
1.6 | - |
acetyl-CoA | mutant delQ284, in presence of 2 mM arginine | Pseudomonas aeruginosa | |
2.4 | - |
acetyl-CoA | wild-type enzyme, in presence of 2 mM arginine | Pseudomonas aeruginosa | |
3 | - |
acetyl-CoA | mutant delE283delQ284 | Pseudomonas aeruginosa | |
7.9 | - |
acetyl-CoA | mutant delQ284 | Pseudomonas aeruginosa | |
10.1 | - |
acetyl-CoA | mutant delE283delQ284, in presence of 2 mM arginine | Pseudomonas aeruginosa | |
15.1 | - |
acetyl-CoA | mutant ins284Ains285A, in presence of 2 mM arginine | Pseudomonas aeruginosa | |
60 | - |
L-glutamate | mutant ins284A | Pseudomonas aeruginosa | |
67 | - |
L-glutamate | mutant ins284Ains285A | Pseudomonas aeruginosa | |
72 | - |
L-glutamate | wild-type enzyme | Pseudomonas aeruginosa | |
78 | - |
L-glutamate | mutant ins284A, in presence of 2 mM arginine | Pseudomonas aeruginosa | |
152 | - |
L-glutamate | mutant ins284Ains285A, in presence of 2 mM arginine | Pseudomonas aeruginosa |
General Information | Comment | Organism |
---|---|---|
physiological function | first enzyme of bacterial and plant arginine biosynthesis, activator of urea cycle in animals | Pseudomonas aeruginosa |