Literature summary for 2.3.1.1 extracted from

Zhao, G.; Haskins, N.; Jin, Z.; M Allewell, N.; Tuchman, M.; Shi, D.
Structure of N-acetyl-L-glutamate synthase/kinase from Maricaulis maris with the allosteric inhibitor L-arginine bound (2013), Biochem. Biophys. Res. Commun., 437, 585-590.

Search for more literature for 2.3.1.1

Activating Compound

Activating Compound	Comment	Organism	Structure
L-arginine	-	Maricaulis maris

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Maricaulis maris

Crystallization (Commentary)

Crystallization (Comment)	Organism
N-acetyl-L-glutamate synthase/kinase in complex with L-arginine, sitting drop vapor diffusion method, using 100 mM sodium cacodylate trihydrate, pH 6.2, 25% (w/v) polypropylene glycol P400 and 200 mM magnesium chloride	Maricaulis maris

Protein Variants

Protein Variants	Comment	Organism
K356H	inactive	Maricaulis maris
N391Q	inactive	Maricaulis maris
R386K	inactive	Maricaulis maris
S387A	the mutant shows reduced activity compared to the wild type enzyme	Maricaulis maris
Y397F	inactive	Maricaulis maris

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
acetyl-CoA + L-glutamate	Maricaulis maris	-	CoA + N-acetyl-L-glutamate	-	?
acetyl-CoA + L-glutamate	Maricaulis maris MCS10	-	CoA + N-acetyl-L-glutamate	-	?

Organism

Organism	UniProt	Comment	Textmining
Maricaulis maris	Q0ASS9	-	-

Purification (Commentary)

Purification (Comment)	Organism
nickel affinity column chromatography and DEAE column chromatography	Maricaulis maris

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
4.97	-	mutant enzyme S387A, at pH 8.5 and 30°C	Maricaulis maris
6.81	-	wild type enzyme, at pH 8.5 and 30°C	Maricaulis maris

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
acetyl-CoA + L-glutamate	-	Maricaulis maris	CoA + N-acetyl-L-glutamate	-	?
acetyl-CoA + L-glutamate	-	Maricaulis maris MCS10	CoA + N-acetyl-L-glutamate	-	?

Synonyms

Synonyms	Comment	Organism
N-acetyl-L-glutamate synthase/kinase	-	Maricaulis maris
NAGS	-	Maricaulis maris

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Release 2023.1 (January 2023)