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Literature summary for 2.3.1.1 extracted from

  • Petri, K.; Walter, F.; Persicke, M.; Rueckert, C.; Kalinowski, J.
    A novel type of N-acetylglutamate synthase is involved in the first step of arginine biosynthesis in Corynebacterium glutamicum (2013), BMC Genomics, 14, 713.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in an Escherichia coli JM109 DELTAargA mutant Corynebacterium glutamicum
gene cg3035, genomic organisation, gene cg3035 has an operon structure together with gene cg3034, expression of gene cg3035 complements an argA-deficient Escherichia coli mutant JM109 strain, recombinant overexpression in Corynebacterium glutamicum strain ATTC 13032 Corynebacterium glutamicum

Protein Variants

Protein Variants Comment Organism
additional information after 48 h at 30°C the mutant DELTAcg3035 exhibits poor growth on unsupplemented MM1 plates in comparison to the Corynebacterium glutamicum wild-type, indicating a bradytrophy of the mutant due to arginine limitation Corynebacterium glutamicum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37200
-
 x * 37200, calculated from amino acid sequence Corynebacterium glutamicum
37200
-
 x * 37200, SDS-PAGE Corynebacterium glutamicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + L-glutamate Corynebacterium glutamicum
-
 CoA + N-acetyl-L-glutamate
-
 ?
acetyl-CoA + L-glutamate Corynebacterium glutamicum ability of protein Cg3035 to acetylate L-glutamate in vitro and in vivo CoA + N-acetyl-L-glutamate
-
 ?
acetyl-CoA + L-glutamate Corynebacterium glutamicum ATCC 13032 ability of protein Cg3035 to acetylate L-glutamate in vitro and in vivo CoA + N-acetyl-L-glutamate
-
 ?
acetyl-CoA + L-glutamate Corynebacterium glutamicum ATCC 13032
-
 CoA + N-acetyl-L-glutamate
-
 ?

Organism

Organism UniProt Comment Textmining
Corynebacterium glutamicum CAF20762 gene cg3035 and gene argA
-
Corynebacterium glutamicum Q8NM40
-
-
Corynebacterium glutamicum ATCC 13032 CAF20762 gene cg3035 and gene argA
-
Corynebacterium glutamicum ATCC 13032 Q8NM40
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + L-glutamate
-
 Corynebacterium glutamicum CoA + N-acetyl-L-glutamate
-
 ?
acetyl-CoA + L-glutamate ability of protein Cg3035 to acetylate L-glutamate in vitro and in vivo Corynebacterium glutamicum CoA + N-acetyl-L-glutamate
-
 ?
acetyl-CoA + L-glutamate ability of protein Cg3035 to acetylate L-glutamate in vitro and in vivo. L-Glutamate most probably is the preferred amino acid substrate for Cg3035 Corynebacterium glutamicum CoA + N-acetyl-L-glutamate
-
 ?
acetyl-CoA + L-glutamate ability of protein Cg3035 to acetylate L-glutamate in vitro and in vivo Corynebacterium glutamicum ATCC 13032 CoA + N-acetyl-L-glutamate
-
 ?
acetyl-CoA + L-glutamate ability of protein Cg3035 to acetylate L-glutamate in vitro and in vivo. L-Glutamate most probably is the preferred amino acid substrate for Cg3035 Corynebacterium glutamicum ATCC 13032 CoA + N-acetyl-L-glutamate
-
 ?
acetyl-CoA + L-glutamate
-
 Corynebacterium glutamicum ATCC 13032 CoA + N-acetyl-L-glutamate
-
 ?

Subunits

Subunits Comment Organism
? x * 37200, calculated from amino acid sequence Corynebacterium glutamicum
? x * 37200, SDS-PAGE Corynebacterium glutamicum

Synonyms

Synonyms Comment Organism
ArgA
-
 Corynebacterium glutamicum
argJ
-
 Corynebacterium glutamicum
Cg3035
-
 Corynebacterium glutamicum
N-acetylglutamate synthase
-
 Corynebacterium glutamicum
NAGS
-
 Corynebacterium glutamicum