Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | no effect | Raphanus sativus | |
EDTA | no effect | Cichorium endivia |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | inhibition at 5 mM | Cichorium endivia | |
Ca2+ | inhibition at 5 mM | Raphanus sativus | |
DTT | concentration above 5 mM results in a marked inhibition of activity | Cichorium endivia | |
DTT | concentration above 5 mM results in a marked inhibition of activity | Raphanus sativus | |
Mg2+ | inhibition at 5 mM | Cichorium endivia | |
Mg2+ | inhibition at 5 mM | Raphanus sativus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
12.5 | - |
caffeoyl-CoA | - |
Cichorium endivia | |
14.3 | - |
4-coumaroyl-CoA | - |
Raphanus sativus | |
14.3 | - |
4-coumaroyl-CoA | - |
Cichorium endivia | |
15.4 | - |
feruloyl-CoA | - |
Cichorium endivia | |
30.5 | - |
caffeoyl-CoA | - |
Raphanus sativus | |
55.5 | - |
feruloyl-CoA | - |
Raphanus sativus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | sligthly stimulated by 3.0 mM, inhibition at 5 mM | Raphanus sativus | |
Ca2+ | sligthly stimulated by 3.0 mM, inhibition at 5 mM | Cichorium endivia | |
Mg2+ | slightly stimulated by 0.5 mM, inhibition at 5 mM | Raphanus sativus | |
Mg2+ | slightly stimulated by 0.5 mM, inhibition at 5 mM | Cichorium endivia |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
gel filtration | Raphanus sativus |
40000 | - |
gel filtration | Cichorium endivia |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-coumaroyl-CoA + shikimate | Raphanus sativus | hydroxycinnamic acid-shikimate ester biosynthesis | CoA + 5-O-(4-coumaroyl)shikimate | - |
? | |
4-coumaroyl-CoA + shikimate | Cichorium endivia | hydroxycinnamic acid-shikimate ester biosynthesis | CoA + 5-O-(4-coumaroyl)shikimate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cichorium endivia | - |
- |
- |
Raphanus sativus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Cichorium endivia |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Raphanus sativus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Cichorium endivia |
Storage Stability | Organism |
---|---|
-20°C, for several months, no apparent loss of activity | Cichorium endivia |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-coumaroyl-CoA + quinate | - |
Raphanus sativus | CoA + 4-coumaroylquinate | - |
? | |
4-coumaroyl-CoA + shikimate | - |
Raphanus sativus | CoA + 5-O-(4-coumaroyl)shikimate | - |
? | |
4-coumaroyl-CoA + shikimate | - |
Cichorium endivia | CoA + 5-O-(4-coumaroyl)shikimate | - |
? | |
4-coumaroyl-CoA + shikimate | hydroxycinnamic acid-shikimate ester biosynthesis | Raphanus sativus | CoA + 5-O-(4-coumaroyl)shikimate | - |
? | |
4-coumaroyl-CoA + shikimate | hydroxycinnamic acid-shikimate ester biosynthesis | Cichorium endivia | CoA + 5-O-(4-coumaroyl)shikimate | - |
? | |
caffeoyl-CoA + shikimate | - |
Raphanus sativus | CoA + 5-O-caffeoylshikimate | - |
? | |
caffeoyl-CoA + shikimate | - |
Cichorium endivia | CoA + 5-O-caffeoylshikimate | - |
? | |
feruloyl-CoA + shikimate | - |
Raphanus sativus | CoA + 5-O-feruloylshikimate | - |
? | |
feruloyl-CoA + shikimate | - |
Cichorium endivia | CoA + 5-O-feruloylshikimate | - |
? | |
additional information | no substrate: cinnamoyl-CoA, L-malate, D(+)-malate, L(+)-tartrate, m-tartrate, citrate, glucose, UDP-glucose, myo-inositol, tyramine, agmatine | Raphanus sativus | ? | - |
? | |
additional information | no substrate: cinnamoyl-CoA, L-malate, D(+)-malate, L(+)-tartrate, m-tartrate, citrate, glucose, UDP-glucose, myo-inositol, tyramine, agmatine | Cichorium endivia | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
50 mM potassium phosphate buffer | Cichorium endivia |
6.5 | - |
pI: 4.33 | Cichorium endivia |
6.8 | - |
50 mM potassium phosphate buffer | Raphanus sativus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | - |
Raphanus sativus |
5 | 9 | - |
Cichorium endivia |