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Literature summary for 2.3.1.138 extracted from
- MYB8 controls inducible phenolamide levels by activating three novel hydroxycinnamoyl-coenzyme A: polyamine transferases in Nicotiana attenuata (2012), Plant Physiol., 158, 389-407.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| AT1 gene, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of C-terminally His6-tagged AT1 in Escherichia coli strain BL21(DE3) | Nicotiana attenuata |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.024 | - |
caffeoyl-CoA | with putrescine, recombinant His-tagged enzyme, pH 7.5, 30°C | Nicotiana attenuata |  |
| 0.664 | - |
putrescine | with caffeoyl-CoA, recombinant His-tagged enzyme, pH 7.5, 30°C | Nicotiana attenuata | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| caffeoyl-CoA + putrescine | Nicotiana attenuata | - |
CoA + N-caffeoylputrescine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Nicotiana attenuata | G9HTF7 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant C-terminally His6-tagged AT1 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis | Nicotiana attenuata |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Nicotiana attenuata | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| caffeoyl-CoA + putrescine | - |
Nicotiana attenuata | CoA + N-caffeoylputrescine | - |
? | |
| caffeoyl-CoA + putrescine | the enzyme is highly specific for putrescine and caffeoyl-CoA | Nicotiana attenuata | CoA + N-caffeoylputrescine | - |
? | |
| feruloyl-CoA + putrescine | feruloyl-CoA gives 10% of the activity with caffeoyl-CoA | Nicotiana attenuata | CoA + N-feruloylputrescine | - |
? | |
| additional information | substrate specificity of recombinant His-tagged AT1, overview. No activity with spermidine, spermine, or 4-coumaroyl-CoA | Nicotiana attenuata | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AT1 | - |
Nicotiana attenuata |
| hydroxycinnamoyl-coenzyme A:putrescine acyltransferase | - |
Nicotiana attenuata |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Nicotiana attenuata |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Nicotiana attenuata |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Nicotiana attenuata | AT1 expression is controlled by the key transcription factor MYB8 | additional information |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | deregulatory effects of transcription factor MYB8 silencing on AT1 control, altered metabolite profile, overview. Silencing of acyltransferase AT1 impairs polyamine accumulation | Nicotiana attenuata |
| metabolism | the enzyme AT1 is involved in a branch of jasmonic acid signaling that defends Nicotiana attenuata plants against herbivores: jasmonic acid, via key transcription factor MYB8's transcriptional control of AT1 and DH29 genes, controls the entire branch of polyamine biosynthesis, overview. AT1, DH29, and CV86 contribute to distinct, but interconnected, MYB8-controlled hydroxycinnamoylputrescine and hydroxycinnamoylspermidine pools | Nicotiana attenuata |
| physiological function | the enzyme is responsible for caffeoylputrescine biosynthesis in tobacco | Nicotiana attenuata |
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