Crystallization (Comment) | Organism |
---|---|
vapor-diffusion method, crystal structure of the enzyme in apo form at 2.33 A resolution, and in complex with UDP-N-acetyl glucosamine and the essential cofactor Mg2+ at 1.96 A resolution. In the crystal, the enzyme forms exact trimers, mainly through contacts between left-handed beta-sheet helix domains. UDP-N-acetylglucosamine and Mg2+ are bound at the uridyltransferase active site, which is in a closed form | Streptococcus pneumoniae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Streptococcus pneumoniae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucosamine 1-phosphate + acetyl-CoA | Streptococcus pneumoniae | the bifunctional enzyme also possesses the activity of EC 2.7.7.23, UDP-N-acetylglucosamine diphosphorylase and performs the last two steps in the synthesis of UDP-N-acetylglucosamine, which is an essential precursor in both the peptidoglycan and the lipopolysaccharide metabolic pathway | N-acetyl-D-glucosamine 1-phosphate + CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus pneumoniae | Q97R46 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucosamine 1-phosphate + acetyl-CoA | the bifunctional enzyme also possesses the activity of EC 2.7.7.23, UDP-N-acetylglucosamine diphosphorylase and performs the last two steps in the synthesis of UDP-N-acetylglucosamine, which is an essential precursor in both the peptidoglycan and the lipopolysaccharide metabolic pathway | Streptococcus pneumoniae | N-acetyl-D-glucosamine 1-phosphate + CoA | - |
? | |
D-glucosamine 1-phosphate + acetyl-CoA | a mechanism is proposed in which the activation energy of the double negatively charged phosphorane transition state is lowered by charge compensation of Mg2+ and the side-chain of Lys22 | Streptococcus pneumoniae | N-acetyl-D-glucosamine 1-phosphate + CoA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GlmU | bifunctional enzyme also possesses the activity of EC 2.7.7.23, UDP-N-acetylglucosamine diphosphorylase | Streptococcus pneumoniae |
N-acetylglucosamine-1-phosphate uridyltransferase | bifunctional enzyme also possesses the activity of EC 2.7.7.23, UDP-N-acetylglucosamine diphosphorylase | Streptococcus pneumoniae |