Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.1.159 extracted from

  • Lukacin, R.; Schreiner, S.; Matern, U.
    Transformation of acridone synthase to chalcone synthase (2001), FEBS Lett., 508, 413-417.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutants in Escherichia coli Ruta graveolens

Protein Variants

Protein Variants Comment Organism
S132T/A133S/V265F site-directed mutagenesis, exchange of 3 residues, responsible for substrate activity, by the corresponding amino acids of chalcone synthase results in transformation of the enzyme to a chalcone synthase, 25fold increased activity, with only 36% remaining acridone synthase activity compared to wild-type Ruta graveolens
V265F site-directed mutagenesis, 75% reduction of ACS catalytic activity, but 2fold increased chalcone synthase activity Ruta graveolens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
structure model of isozyme II Ruta graveolens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
malonyl-CoA + N-methylanthraniloyl-CoA Ruta graveolens
-
CoA + 1,3-dihydroxy-N-methylacridone + CO2
-
ir

Organism

Organism UniProt Comment Textmining
Ruta graveolens
-
2 isozymes I and II
-

Reaction

Reaction Comment Organism Reaction ID
3 malonyl-CoA + N-methylanthraniloyl-CoA = 4 CoA + 1,3-dihydroxy-N-methylacridone + 3 CO2 + H2O two-step mechanism Ruta graveolens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
malonyl-CoA + 4-coumaroyl-CoA wild-type shows low activity, while mutants show highly increased activity Ruta graveolens CoA + naringenin chalcone
-
?
malonyl-CoA + N-methylanthraniloyl-CoA
-
Ruta graveolens CoA + 1,3-dihydroxy-N-methylacridone + CO2
-
ir

Synonyms

Synonyms Comment Organism
ACS
-
Ruta graveolens