Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.1.161 extracted from

  • Ma, S.M.; Tang, Y.
    Biochemical characterization of the minimal polyketide synthase domains in the lovastatin nonaketide synthase LovB (2007), FEBS J., 274, 2854-2864.
    View publication on PubMed
Search for more literature for 2.3.1.161

Cloned(Commentary)

Cloned (Comment) Organism
minimal polyketide synthase domains of subunit LovB as standalone proteins Aspergillus terreus
the entire lovB gene is inserted into pET21a+ to create pSMa33, the LovB KS-MAT didomain, MAT, ACP, ACP-CON and CON are each amplified from pSMa33 and cloned into pET28a+ Aspergillus terreus

Protein Variants

Protein Variants Comment Organism
additional information expression of minimal polyketide synthase domains of subunit LovB as standalone proteins. The didomain proteins ketosynthase-malonyl-coenzyme A:acyl carrier protein acyltransferase KS-MAT can transfer the acyl group to both the cognate LovB acyl carrier protein and heterologous acyl carrier proteins from bacterial type I and type II polyketide synthases. It transfers malonyl and acetyl groups with kcat/Km values of 0.62 per min and 0.032 per min, resp Aspergillus terreus
S208A LovB MAT mutant Aspergillus terreus
S209A LovB MAT mutant Aspergillus terreus
S656A inactivation of malonyl-coenzyme A:acyl carrier protein acyltransferase domain in ketosynthase-malonyl-coenzyme A:acyl carrier protein acyltransferase for assay of ketosynthase activity. Ketosynthase displays no activity towards acetyl groups but recognizes malonyl groups in the absence of cerulenin Aspergillus terreus
S656A LovB KS-MAT mutant, MAT domain inactivated Aspergillus terreus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
11000
-
 LovB ACP, determined by SDS-PAGE Aspergillus terreus
52000
-
 LovB MAT, determined by SDS-PAGE Aspergillus terreus
66000
-
 LovB ACP-CON, determined by SDS-PAGE Aspergillus terreus
103000
-
 LovB KS-MAT, determined by SDS-PAGE Aspergillus terreus
335000
-
 LovB Aspergillus terreus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + malonyl-CoA + NADPH + H+ + S-adenosyl-L-methionine Aspergillus terreus
-
 dihydromonacolin L + CoA + CO2 + NADP+ + S-adenosyl-L-homocysteine + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus terreus
-
-
-
Aspergillus terreus Q9Y8A5
-
-

Purification (Commentary)

Purification (Comment) Organism
the single domains are purified using Ni-nitrilotriacetic agarose resin and anion-exchange chromatography Aspergillus terreus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + malonyl-CoA + NADPH + H+ + S-adenosyl-L-methionine
-
 Aspergillus terreus dihydromonacolin L + CoA + CO2 + NADP+ + S-adenosyl-L-homocysteine + H2O
-
 ?

Synonyms

Synonyms Comment Organism
lovastatin nonaketide synthase
-
 Aspergillus terreus
LovB
-
 Aspergillus terreus