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Literature summary for 2.3.1.162 extracted from

  • Walker, K.; Ketchum, R.E.; Hezari, M.; Gatfield, D.; Goleniowski, M.; Barthol, A.; Croteau, R.
    Partial purification and characterization of acetyl coenzyme A: taxa-4(20),11(12)-dien-5alpha-ol O-acetyl transferase that catalyzes the first acylation step of taxol biosynthesis (1999), Arch. Biochem. Biophys., 364, 273-279.
    View publication on PubMed
Search for more literature for 2.3.1.162

Application

Application Comment Organism
medicine understanding of biosynthetic pathway of taxol, target: possibly biological production of taxol, taxol or paclitaxel is now well established as a potent chemotherapeutic drug Taxus cuspidata
medicine understanding of biosynthetic pathway of taxol, target: possibly biological production of taxol, taxol or paclitaxel is now well established as a potent chemotherapeutic drug Taxus canadensis

Inhibitors

Inhibitors Comment Organism Structure
CoA weak Taxus canadensis
CoA weak Taxus cuspidata
N-ethylmaleimide weak Taxus canadensis
N-ethylmaleimide weak Taxus cuspidata
p-hydroxymercuribenzoate weak Taxus canadensis
p-hydroxymercuribenzoate weak Taxus cuspidata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0042
-
 taxa-4(20),11-dien-5alpha-ol
-
 Taxus cuspidata
0.0042
-
 taxa-4(20),11-dien-5alpha-ol
-
 Taxus canadensis
0.0055
-
 acetyl-CoA
-
 Taxus cuspidata
0.0055
-
 acetyl-CoA
-
 Taxus canadensis

Metals/Ions

Metals/Ions Comment Organism Structure
additional information monovalent and divalent cations show no effect on the enzyme Taxus cuspidata
additional information monovalent and divalent cations show no effect on the enzyme Taxus canadensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 gel chromatography Taxus cuspidata
50000
-
 gel chromatography Taxus canadensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Taxus cuspidata catalyzes the first acylation step of taxol biosynthesis ?
-
 ?
additional information Taxus canadensis catalyzes the first acylation step of taxol biosynthesis ?
-
 ?

Organism

Organism UniProt Comment Textmining
Taxus canadensis
-
 methyl jasmonate-induced cells
-
Taxus cuspidata
-
 methyl jasmonate-induced cells
-

Purification (Commentary)

Purification (Comment) Organism
partially Taxus cuspidata
partially Taxus canadensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + farnesol
-
 Taxus cuspidata CoA + ?
-
 ?
acetyl-CoA + geranylgeraniol
-
 Taxus cuspidata CoA + ?
-
 ?
acetyl-CoA + phytol
-
 Taxus cuspidata CoA + ?
-
 ?
acetyl-CoA + taxa-4(20),11(12)-dien-5alpha-ol
-
 Taxus cuspidata CoA + taxa-4(20),11-dien-5alpha-yl acetate
-
 ?
acetyl-CoA + taxa-4(20),11(12)-dien-5alpha-ol
-
 Taxus canadensis CoA + taxa-4(20),11-dien-5alpha-yl acetate
-
 ?
acetyl-CoA + taxadienol
-
 Taxus cuspidata CoA + ?
-
 ?
additional information catalyzes the first acylation step of taxol biosynthesis Taxus cuspidata ?
-
 ?
additional information catalyzes the first acylation step of taxol biosynthesis Taxus canadensis ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
 pI: 4.7 Taxus cuspidata
9
-
 pI: 4.7 Taxus canadensis

pH Range

pH Minimum pH Maximum Comment Organism
7.5 10 50% of maximal activity at pH 7.50 and pH 10.0, enzyme inactive at pH 11.0 Taxus cuspidata
7.5 10 50% of maximal activity at pH 7.50 and pH 10.0, enzyme inactive at pH 11.0 Taxus canadensis