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Literature summary for 2.3.1.167 extracted from

  • Guo, B.; Kai, G.; Gong, Y.; Jin, H.; Wang, Y.; Miao, Z.; Sun, X.; Tang, K.
    Molecular cloning and heterologous expression of a 10-deacetylbaccatin III-10-O-acetyl transferase cDNA from Taxus x media (2007), Mol. Biol. Rep., 34, 89-95.
    View publication on PubMed
Search for more literature for 2.3.1.167

Activating Compound

Activating Compound Comment Organism Structure
ammonium ceric(IV) sulfate
-
 Taxus x media
methyl jasmonate
-
 Taxus x media
Silver nitrate
-
 Taxus x media

Cloned(Commentary)

Cloned (Comment) Organism
the amplified PCR fragment is subcloned into the pGEM-T Easy vector and subsequently into the pET32a+ vector for expression in Escherichia coli BL21DE3 cells Taxus x media

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
16000
-
 tag-protein Taxus x media
48980
-
 predicted molecular mass Taxus x media
65000
-
 determined by SDS-PAGE, TmDBAT plus fused tag Taxus x media

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + 10-deacetylbaccatin III Taxus x media
-
 CoA + baccatin III
-
 ?

Organism

Organism UniProt Comment Textmining
Taxus x media Q6SQJ5
-
-

Purification (Commentary)

Purification (Comment) Organism
by affinity chromatography in Ni-nitrilotriacetic acid columns, the tag is removed by digestion with enterokinase Taxus x media

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Taxus x media
-
stem
-
 Taxus x media
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + 10-deacetylbaccatin III
-
 Taxus x media CoA + baccatin III
-
 ?

Synonyms

Synonyms Comment Organism
10-deacetylbaccatin III-10-O-acetyl transferase
-
 Taxus x media
TmDBAT
-
 Taxus x media

pI Value

Organism Comment pI Value Maximum pI Value
Taxus x media predicted pI-value
-
 6.05