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Literature summary for 2.3.1.169 extracted from

  • Murakami, E.; Ragsdale, S.W.
    Evidence for intersubunit communication during acetyl-CoA cleavage by the multienzyme CO dehydrogenase/acetyl-CoA synthase complex from Methanosarcina thermophila. Evidence that the beta subunit catalyzes C-C and C-S bond cleavage (2000), J. Biol. Chem., 275, 4699-4707.
    View publication on PubMed
Search for more literature for 2.3.1.169

Metals/Ions

Metals/Ions Comment Organism Structure
Fe corrinoid/iron-sulfur protein required Methanosarcina thermophila
Fe a Ni/Fe-S cluster of multienzyme CO dehydrogenase/acetyl-CoA synthase complex is the active site of acetyl-CoA cleavage and synthesis Methanosarcina thermophila
Ni a Ni/Fe-S cluster of multienzyme CO dehydrogenase/acetyl-CoA synthase complex is the active site of acetyl-CoA cleavage and synthesis Methanosarcina thermophila

Organism

Organism UniProt Comment Textmining
Methanosarcina thermophila P72021 TM1
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Methanosarcina thermophila TM1 P72021 TM1
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CH3-CO-S-CoA + H+ + tetrahydromethanopterin
-
 Methanosarcina thermophila CH3-tetrahydromethanopterin + CO + HS-CoA
-
 ?
CH3-CO-S-CoA + H+ + tetrahydromethanopterin
-
 Methanosarcina thermophila TM1 CH3-tetrahydromethanopterin + CO + HS-CoA
-
 ?
CO + H2O the multienzyme complex catalyses the reversible oxidation of CO to CO2 Methanosarcina thermophila CO2 + H+ + electron
-
 r
CO + H2O the multienzyme complex catalyses the reversible oxidation of CO to CO2 Methanosarcina thermophila TM1 CO2 + H+ + electron
-
 r
additional information the multienzyme complex catalyses the exchange between free CO and carbonyl group of acetyl-CoA, and the exchange between CoA and the CoA moiety of acetyl-CoA Methanosarcina thermophila ?
-
 ?
additional information the multienzyme complex catalyses the exchange between free CO and carbonyl group of acetyl-CoA, and the exchange between CoA and the CoA moiety of acetyl-CoA Methanosarcina thermophila TM1 ?
-
 ?

Subunits

Subunits Comment Organism
More alpha,beta,gamma,delta,epsilon, the enzyme complex is part of a five-subunit complex, the alpha and epsilon subunits are required for CO oxidation, the gamma end delta subunits constitute a corrinoid/iron-sulfur protein, the beta subunit harbors the Ni/Fe-S cluster, that is the active site of acetyl-CoA cleavage/synthesis, the interaction between the alpha,epsilon dimer and the beta subunit is necessary for breaking and forming the C-C bond of acetyl-CoA, SDS-PAGE Methanosarcina thermophila