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Literature summary for 2.3.1.171 extracted from
- Identification of a cDNA encoding malonyl-coenzyme A:anthocyanidin 3-O-glucoside 6''-O-malonyltransferase from cineraria (Senecio cruentus) flowers (2003), Plant Biotechnol., 20, 229-234.
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| agriculture | the enzyme may serve as a tool for the metabolic engineering of flavonoid biosynthesis leading to the accumulation of cinerarin in flowers, which results in the coloration of blue flowers | Pericallis cruenta |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli JM109 under the control of T5 promoter as an in-frame N-terminal fusion with a His6 tag | Pericallis cruenta |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| acetyl-CoA | 0.1 mM, 63% inhibition | Pericallis cruenta |  |
| CdCl2 | 0.1 mM, 48% loss of activity | Pericallis cruenta | |
| CoA | 0.1 mM, 67% inhibition | Pericallis cruenta | |
| CuCl2 | 0.1 mM, complete inhibition | Pericallis cruenta | |
| HgCl2 | 0.1 mM, complete inhibition | Pericallis cruenta | |
| MgCl2 | 0.1 mM, 52% inhibition | Pericallis cruenta | |
| additional information | 0.1 mM EDTA or 0.1 mM CaCl2, CoCl2, FeCl2, MnCl2, NiCl2 or SnCl2 have negligible effects on the enzyme activity | Pericallis cruenta | |
| NEM | 5 mM, complete inhibition | Pericallis cruenta | |
| ZnCl2 | 0.1 mM, 66% inhibition | Pericallis cruenta | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.011 | - |
pelargonidin 3-O-beta-D-glucoside | - |
Pericallis cruenta | |
| 0.061 | - |
malonyl-CoA | - |
Pericallis cruenta | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| malonyl-CoA + delphinidin 3-O-beta-D-glucoside | Pericallis cruenta | because the reaction product delphinidin 3-O-6'-malonylglucoside, is a part of cinerarin biosynthesis, it is highly likely that Sc3MaT is involved in the biosynthesis of cinerarin | CoA + delphinidin 3-O-(6-malonyl-beta-D-glucoside) | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pericallis cruenta | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| flower | - |
Pericallis cruenta | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| malonyl-CoA + cyanidin 3-O-beta-D-glucoside | 110% of the activity with pelargonidin 3-O-glucoside | Pericallis cruenta | CoA + cyanidin 3-O-(6-O-malonyl-beta-D-glucoside) | - |
? | |
| malonyl-CoA + delphinidin 3-O-beta-D-glucoside | because the reaction product delphinidin 3-O-6'-malonylglucoside, is a part of cinerarin biosynthesis, it is highly likely that Sc3MaT is involved in the biosynthesis of cinerarin | Pericallis cruenta | CoA + delphinidin 3-O-(6-malonyl-beta-D-glucoside) | - |
? | |
| malonyl-CoA + delphinidin 3-O-beta-D-glucoside | 153% of the activity with pelargonidin 3-O-glucoside | Pericallis cruenta | CoA + delphinidin 3-O-(6-O-malonyl-beta-D-glucoside) | - |
? | |
| malonyl-CoA + pelargonidin 3-O-beta-D-glucoside | - |
Pericallis cruenta | CoA + pelargonidin 3-O-(6-O-malonyl-beta-D-glucoside) | - |
? | |
| methylmalonyl-CoA + pelargonidin 3-O-beta-D-glucoside | 15% of the activity with methylmalonyl-CoA | Pericallis cruenta | CoA + pelargonidin 3-O-(6-O-methylmalonyl-beta-D-glucoside) | - |
? | |
| succinyl-CoA + pelargonidin 3-O-beta-D-glucoside | 20% of the activity with methylmalonyl-CoA | Pericallis cruenta | CoA + pelargonidin 3-O-(6-O-succinyl-beta-D-glucoside) | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 3MaT | - |
Pericallis cruenta |
| anthocyanidin 3-glucoside malonyltransferase | - |
Pericallis cruenta |
| anthocyanin 3-glucoside malonyltransferase | - |
Pericallis cruenta |
| Dv3MaT | - |
Pericallis cruenta |
| malonyl-CoA:anthocyanin 3-O-glucoside-6'-O-malonyltransferase | - |
Pericallis cruenta |
| malonyl-coenzyme A:anthocyanidin 3-O-glucoside 6''-O-malonyltransferase | - |
Pericallis cruenta |
| malonyl-coenzyme A:anthocyanidin-3-O-beta-D-glucoside 6''-O-malonyltransferase | - |
Pericallis cruenta |
| malonyltransferase, anthocyanidin 3-glucoside | - |
Pericallis cruenta |
| Sc3MaT | - |
Pericallis cruenta |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
pH 7.0, 20 min, stable below | Pericallis cruenta |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 8.8 | - |
malonyl-CoA | - |
Pericallis cruenta | |
| 8.8 | - |
pelargonidin 3-O-beta-D-glucoside | - |
Pericallis cruenta | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Pericallis cruenta |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 7.5 | pH 7.0, 20 min, stable | Pericallis cruenta |
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Release 2023.1 (January 2023)
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