Cloned (Comment) | Organism |
---|---|
expression of the His-tagged enzyme in Escherichia coli strain BL21 (DE3) | Methylotuvimicrobium alcaliphilum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | - |
Methylophaga alcalica | |
Cd2+ | - |
Methylophaga thalassica | |
Cd2+ | - |
Methylotuvimicrobium alcaliphilum | |
Cu2+ | - |
Methylophaga alcalica | |
Cu2+ | - |
Methylophaga thalassica | |
Zn2+ | - |
Methylophaga alcalica | |
Zn2+ | - |
Methylophaga thalassica | |
Zn2+ | - |
Methylotuvimicrobium alcaliphilum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
acetyl-CoA | pH 9.5, 25°C | Methylophaga alcalica | |
0.037 | - |
acetyl-CoA | pH 9.5, 25°C | Methylotuvimicrobium alcaliphilum | |
0.076 | - |
acetyl-CoA | pH 9.5, 25°C | Methylophaga thalassica | |
0.365 | - |
L-2,4-diaminobutanoate | pH 9.5, 25°C | Methylophaga thalassica | |
0.375 | - |
L-2,4-diaminobutanoate | pH 9.5, 25°C | Methylophaga alcalica | |
0.465 | - |
L-2,4-diaminobutanoate | pH 9.5, 25°C | Methylotuvimicrobium alcaliphilum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | activates, best at 0.25 M | Methylotuvimicrobium alcaliphilum | |
additional information | no activation by NaCl and KCl | Methylophaga thalassica | |
additional information | no activation by NaCl and KCl | Methylophaga alcalica | |
NaCl | activates, best at 0.1-0.2 M | Methylotuvimicrobium alcaliphilum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20000 | - |
2 * 20000, recombinant His-tagged enzyme, SDS-PAGE | Methylophaga thalassica |
20000 | - |
2 * 20000, recombinant His-tagged enzyme, SDS-PAGE | Methylotuvimicrobium alcaliphilum |
20000 | - |
2 * 20000, recombinant His-tagged enzyme, SDS-PAGE | Methylophaga alcalica |
40000 | - |
recombinant His-tagged enzyme, gel filtration | Methylophaga thalassica |
40000 | - |
recombinant His-tagged enzyme, gel filtration | Methylotuvimicrobium alcaliphilum |
40000 | - |
recombinant His-tagged enzyme, gel filtration | Methylophaga alcalica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + L-2,4-diaminobutanoate | Methylophaga thalassica | - |
CoA + N4-acetyl-L-2,4-diaminobutanoate | - |
? | |
acetyl-CoA + L-2,4-diaminobutanoate | Halomonas elongata | - |
CoA + N4-acetyl-L-2,4-diaminobutanoate | - |
? | |
acetyl-CoA + L-2,4-diaminobutanoate | Methylotuvimicrobium alcaliphilum | - |
CoA + N4-acetyl-L-2,4-diaminobutanoate | - |
? | |
acetyl-CoA + L-2,4-diaminobutanoate | Methylophaga alcalica | - |
CoA + N4-acetyl-L-2,4-diaminobutanoate | - |
? | |
acetyl-CoA + L-2,4-diaminobutanoate | Methylotuvimicrobium alcaliphilum 20Z | - |
CoA + N4-acetyl-L-2,4-diaminobutanoate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Halomonas elongata | - |
- |
- |
Methylophaga alcalica | - |
gene ectA | - |
Methylophaga thalassica | - |
gene ectA | - |
Methylotuvimicrobium alcaliphilum | - |
gene ectA | - |
Methylotuvimicrobium alcaliphilum 20Z | - |
gene ectA | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography | Methylotuvimicrobium alcaliphilum |
Storage Stability | Organism |
---|---|
4-70°C, purified recombinant His-tagged enzyme, in 50 mM Tris-HCl, pH 8.5, stable at least for 1 month at protein concentration 0.5 mg/ml | Methylophaga thalassica |
4-70°C, purified recombinant His-tagged enzyme, in 50 mM Tris-HCl, pH 8.5, stable at least for 1 month at protein concentration 0.5 mg/ml | Methylotuvimicrobium alcaliphilum |
4-70°C, purified recombinant His-tagged enzyme, in 50 mM Tris-HCl, pH 8.5, stable at least for 1 month at protein concentration 0.5 mg/ml | Methylophaga alcalica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + L-2,4-diaminobutanoate | - |
Methylophaga thalassica | CoA + N4-acetyl-L-2,4-diaminobutanoate | - |
? | |
acetyl-CoA + L-2,4-diaminobutanoate | - |
Halomonas elongata | CoA + N4-acetyl-L-2,4-diaminobutanoate | - |
? | |
acetyl-CoA + L-2,4-diaminobutanoate | - |
Methylotuvimicrobium alcaliphilum | CoA + N4-acetyl-L-2,4-diaminobutanoate | - |
? | |
acetyl-CoA + L-2,4-diaminobutanoate | - |
Methylophaga alcalica | CoA + N4-acetyl-L-2,4-diaminobutanoate | - |
? | |
acetyl-CoA + L-2,4-diaminobutanoate | - |
Methylotuvimicrobium alcaliphilum 20Z | CoA + N4-acetyl-L-2,4-diaminobutanoate | - |
? | |
additional information | usage of 5,5-dithio-bis-(2-nitrobenzoic acid) for detection of activity | Methylophaga thalassica | ? | - |
? | |
additional information | usage of 5,5-dithio-bis-(2-nitrobenzoic acid) for detection of activity | Halomonas elongata | ? | - |
? | |
additional information | usage of 5,5-dithio-bis-(2-nitrobenzoic acid) for detection of activity | Methylotuvimicrobium alcaliphilum | ? | - |
? | |
additional information | usage of 5,5-dithio-bis-(2-nitrobenzoic acid) for detection of activity | Methylophaga alcalica | ? | - |
? | |
additional information | usage of 5,5-dithio-bis-(2-nitrobenzoic acid) for detection of activity | Methylotuvimicrobium alcaliphilum 20Z | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 20000, recombinant His-tagged enzyme, SDS-PAGE | Methylophaga thalassica |
homodimer | 2 * 20000, recombinant His-tagged enzyme, SDS-PAGE | Methylotuvimicrobium alcaliphilum |
homodimer | 2 * 20000, recombinant His-tagged enzyme, SDS-PAGE | Methylophaga alcalica |
Synonyms | Comment | Organism |
---|---|---|
DABA acetyltransferase | - |
Methylophaga thalassica |
DABA acetyltransferase | - |
Halomonas elongata |
DABA acetyltransferase | - |
Methylotuvimicrobium alcaliphilum |
DABA acetyltransferase | - |
Methylophaga alcalica |
EctA | - |
Methylophaga thalassica |
EctA | - |
Halomonas elongata |
EctA | - |
Methylotuvimicrobium alcaliphilum |
EctA | - |
Methylophaga alcalica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
- |
Methylotuvimicrobium alcaliphilum |
25 | - |
assay at | Halomonas elongata |
30 | 35 | - |
Methylophaga thalassica |
30 | 35 | - |
Methylophaga alcalica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | - |
- |
Halomonas elongata |
8.5 | - |
- |
Methylophaga thalassica |
9.5 | - |
- |
Methylotuvimicrobium alcaliphilum |
9.5 | - |
- |
Methylophaga alcalica |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in the pathway of ectoine biosynthesis | Methylophaga thalassica |
metabolism | the enzyme is involved in the pathway of ectoine biosynthesis | Halomonas elongata |
metabolism | the enzyme is involved in the pathway of ectoine biosynthesis | Methylotuvimicrobium alcaliphilum |
metabolism | the enzyme is involved in the pathway of ectoine biosynthesis | Methylophaga alcalica |