Cloned (Comment) | Organism |
---|---|
gene SACOL2570, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21-CodonPlus(DE3)RIPL | Staphylococcus aureus |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme bound with CoA, hanging drop vapor diffusion method, mixing f 0.001 ml of protein solution containing 2.6 mg/ml Se-Met-labeled enzyme in 10 mM HEPES buffer, pH 7.5 and 500 mM NaCl, with 0.001 ml of reervoir solution containing 200 mM di-ammonium hydrogen citrate, 20% w/v PEG 3350, pH 5.0, 22°C, X-ray diffraction structure determination and analysis at 1.6 A resolution, molecular dynamics and mechanics simulation, modeling | Staphylococcus aureus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + a beta-D-galactoside | Staphylococcus aureus | galactoside acetyltransferases are enzymes that transfer an acetyl group from acetyl coen-zyme A to beta-galactosides. The enzymes have a broad substrate specificity and can acetylate many galactoside derivatives, including thiogalactosides and lactosides | CoA + a 6-acetyl-beta-D-galactoside | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | Q5HCZ5 | subsp. COL, methicillin-resistant strain, gene SACOL2570 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21-CodonPlus(DE3)RIPL by nickel affinity chromatography, tag cleavage with TEV protease, another step of nickel affinity chromatography, and gel filtration | Staphylococcus aureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + a beta-D-galactoside | galactoside acetyltransferases are enzymes that transfer an acetyl group from acetyl coen-zyme A to beta-galactosides. The enzymes have a broad substrate specificity and can acetylate many galactoside derivatives, including thiogalactosides and lactosides | Staphylococcus aureus | CoA + a 6-acetyl-beta-D-galactoside | - |
? | |
acetyl-CoA + a beta-D-galactoside | CoA binds in the active site formed by the residues from adjacent LbetaH domains. After determination of CoA-bound structure, molecular dynamics simulations are performed to model the binding of AcCoA. Binding of both AcCoA and CoA to SACOL2570 is verified by isothermal titration calorimetry | Staphylococcus aureus | CoA + a 6-acetyl-beta-D-galactoside | binding of CoA in the 3V4E crystal structure involves residues from two adjacent monomers in the homotrimeric assembly. Residues responsible for CoA binding by SACOL2570 are Asn84, Ala112, Gly141, Lys165, and Arg182 | ? |
Subunits | Comment | Organism |
---|---|---|
heterotrimer | in solution, small-angle X-ray scattering and dynamic light scattering. The protein subunit consists of an N-terminal alpha-helical domain connected to a C-terminal LbetaH domain | Staphylococcus aureus |
Synonyms | Comment | Organism |
---|---|---|
galactoside acetyltransferase | - |
Staphylococcus aureus |
GAT | - |
Staphylococcus aureus |
SACOL2570 | - |
Staphylococcus aureus |
Organism | Comment | Expression |
---|---|---|
Staphylococcus aureus | the enzyme is downregulated by fusidic acid | down |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme is a member of the galactoside acetyltransferase superfamily from methicillin-resistant Staphylococcus aureus | Staphylococcus aureus |
physiological function | the enzyme SACOL2570 is a putative galactoside O-acetyltransferase (GAT) protein and may act as a detoxifying enzyme, acetylating nonmetabolizable carbohydrates to prevent their reentry into the cell. It is potentially involved in the cellular processes of toxin production and antibiotic resistance | Staphylococcus aureus |