Application | Comment | Organism |
---|---|---|
medicine | the enzyme is a target of antibacterial treatment of the pathogen Staphylococcus aureus | Staphylococcus aureus |
pharmacology | the enzyme is a target for development of inhibitors for treatment of the multi-drug resistant pathogen Staphylococcus aureus | Staphylococcus aureus |
Cloned (Comment) | Organism |
---|---|
gene fabH, overexpression of N-terminal His-tagged enzyme in Escherichia coli strain BL21(DE3) | Staphylococcus aureus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-phenyl-5-phenylimino-[1,2,4]dithiazolidin-3-one | CAS-No. 5338-82-9, IC50 is 0.00078 mM | Staphylococcus aureus | |
5-chloro-4-phenyl-[1,2]-dithiol-3-one | CAS-No. 2425-05-0, IC50 is 0.0019 mM | Staphylococcus aureus | |
cerulenin | - |
Staphylococcus aureus | |
additional information | antibacterial potency of inhibitors, overview | Staphylococcus aureus | |
thiolactomycin | weak inhibition, IC50 is above 0.1 mM | Staphylococcus aureus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0003 | - |
isobutyryl-CoA | pH 7.2, 30°C, recombinant enzyme | Staphylococcus aureus | |
0.0023 | - |
butyryl-CoA | pH 7.2, 30°C, recombinant enzyme | Staphylococcus aureus | |
0.0062 | - |
acetyl-CoA | pH 7.2, 30°C, recombinant enzyme | Staphylococcus aureus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
2 * 37000, recombinant enzyme, SDS-PAGE | Staphylococcus aureus |
66700 | - |
recombinant enzyme, gel filtration | Staphylococcus aureus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + malonyl-[acyl-carrier protein] | Staphylococcus aureus | the enzyme is involved in initiation of fatty acid elogantion by the fatty acid synthase II catalyzing the decarboxylative condensation between malonyl-ACP and acetyl-CoA, the enzyme is essential for viability, overview | acetoacetyl-[acyl-carrier protein] + CoA + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | - |
single-copy gene fabH | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
no modification | strain MRSA, N315, and strain VRSA, Mu 50 | Staphylococcus aureus |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration | Staphylococcus aureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + malonyl-[acyl-carrier protein] | - |
Staphylococcus aureus | acetoacetyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
acetyl-CoA + malonyl-[acyl-carrier protein] | the enzyme is involved in initiation of fatty acid elogantion by the fatty acid synthase II catalyzing the decarboxylative condensation between malonyl-ACP and acetyl-CoA, the enzyme is essential for viability, overview | Staphylococcus aureus | acetoacetyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
butyryl-CoA + malonyl-[acyl-carrier protein] | - |
Staphylococcus aureus | 3-oxo-hexanoyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
isobutyryl-CoA + malonyl-[acyl-carrier protein] | isobutyryl-CoA is the preferred CoA primer substrate | Staphylococcus aureus | 3-oxo-4-methyl-pentanoyl-[acyl-carrier protein] + CoA + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 37000, recombinant enzyme, SDS-PAGE | Staphylococcus aureus |
Synonyms | Comment | Organism |
---|---|---|
ACP synthase III | - |
Staphylococcus aureus |
beta-ketoacyl-acyl carrier protein synthase III | - |
Staphylococcus aureus |
FabH | - |
Staphylococcus aureus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Staphylococcus aureus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.27 | - |
acetyl-CoA | pH 7.2, 30°C, recombinant enzyme | Staphylococcus aureus | |
0.72 | - |
butyryl-CoA | pH 7.2, 30°C, recombinant enzyme | Staphylococcus aureus | |
1.63 | - |
isobutyryl-CoA | pH 7.2, 30°C, recombinant enzyme | Staphylococcus aureus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Staphylococcus aureus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00078 | - |
CAS-No. 5338-82-9, IC50 is 0.00078 mM | Staphylococcus aureus | 4-phenyl-5-phenylimino-[1,2,4]dithiazolidin-3-one | |
0.0019 | - |
CAS-No. 2425-05-0, IC50 is 0.0019 mM | Staphylococcus aureus | 5-chloro-4-phenyl-[1,2]-dithiol-3-one | |
0.1 | - |
weak inhibition, IC50 is above 0.1 mM | Staphylococcus aureus | thiolactomycin |