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Literature summary for 2.3.1.180 extracted from
- Coexpression of genetically engineered 3-ketoacyl-ACP synthase III (fabH) and polyhydroxyalkanoate synthase (phaC) genes leads to short-chain-length-medium-chain-length polyhydroxyalkanoate copolymer production from glucose in Escherichia coli JM109 (2004), Appl. Environ. Microbiol., 70, 999-1007.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of wild-type and mutant enzymes in strain JM109, coexpression with gene phaC from Aeromonas caviae and gene phaC1 from Pseudomonas sp. strain 61-3 | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| F87A | site-directed mutagenesis, coexpression of the mutant enzyme with the phaC gene from Aeromonas caviae in a recombinant strain confers the ability to synthesize polyhydroxyalkanoates, coexpression of the mutant with gene phaC1 from Pseudomonas sp. strain 61-3 in Escherichia coli confers the ability to synthesize polyhydroxyalkanoates, overview | Escherichia coli |
| F87C | site-directed mutagenesis, coexpression of the mutant enzyme with the phaC gene from Aeromonas caviae in a recombinant strain confers the ability to synthesize polyhydroxyalkanoates, coexpression of the mutant with gene phaC1 from Pseudomonas sp. strain 61-3 in Escherichia coli confers the ability to synthesize polyhydroxyalkanoates, overview | Escherichia coli |
| F87D | site-directed mutagenesis, coexpression of the mutant enzyme with the phaC gene from Aeromonas caviae in a recombinant strain does not confer the ability to synthesize polyhydroxyalkanoates, coexpression of the mutant with gene phaC1 from Pseudomonas sp. strain 61-3 in Escherichia coli does not confer the ability to synthesize polyhydroxyalkanoates, overview | Escherichia coli |
| F87E | site-directed mutagenesis, coexpression of the mutant enzyme with the phaC gene from Aeromonas caviae in a recombinant strain does not confer the ability to synthesize polyhydroxyalkanoates, coexpression of the mutant with gene phaC1 from Pseudomonas sp. strain 61-3 in Escherichia coli does not confer the ability to synthesize polyhydroxyalkanoates, overview | Escherichia coli |
| F87G | site-directed mutagenesis, coexpression of the mutant enzyme with the phaC gene from Aeromonas caviae in a recombinant strain does not confer the ability to synthesize polyhydroxyalkanoates, coexpression of the mutant with gene phaC1 from Pseudomonas sp. strain 61-3 in Escherichia coli does not confer the ability to synthesize polyhydroxyalkanoates, overview | Escherichia coli |
| F87H | site-directed mutagenesis, coexpression of the mutant enzyme with the phaC gene from Aeromonas caviae in a recombinant strain does not confer the ability to synthesize polyhydroxyalkanoates, coexpression of the mutant with gene phaC1 from Pseudomonas sp. strain 61-3 in Escherichia coli does not confer the ability to synthesize polyhydroxyalkanoates, overview | Escherichia coli |
| F87I | site-directed mutagenesis, coexpression of the mutant enzyme with the phaC gene from Aeromonas caviae in a recombinant strain confers the ability to synthesize polyhydroxyalkanoates, coexpression of the mutant with gene phaC1 from Pseudomonas sp. strain 61-3 in Escherichia coli confers the ability to synthesize polyhydroxyalkanoates, overview | Escherichia coli |
| F87K | site-directed mutagenesis, coexpression of the mutant enzyme with the phaC gene from Aeromonas caviae in a recombinant strain does not confer the ability to synthesize polyhydroxyalkanoates, coexpression of the mutant with gene phaC1 from Pseudomonas sp. strain 61-3 in Escherichia coli does not confer the ability to synthesize polyhydroxyalkanoates, overview | Escherichia coli |
| F87L | site-directed mutagenesis, coexpression of the mutant enzyme with the phaC gene from Aeromonas caviae in a recombinant strain confers the ability to synthesize polyhydroxyalkanoates, coexpression of the mutant with gene phaC1 from Pseudomonas sp. strain 61-3 in Escherichia coli confers the ability to synthesize polyhydroxyalkanoates, overview | Escherichia coli |
| F87M | site-directed mutagenesis, coexpression of the mutant enzyme with the phaC gene from Aeromonas caviae in a recombinant strain confers the ability to synthesize polyhydroxyalkanoates, coexpression of the mutant with gene phaC1 from Pseudomonas sp. strain 61-3 in Escherichia coli confers the ability to synthesize polyhydroxyalkanoates, overview | Escherichia coli |
| F87N | site-directed mutagenesis, coexpression of the mutant enzyme with the phaC gene from Aeromonas caviae in a recombinant strain does not confer the ability to synthesize polyhydroxyalkanoates, coexpression of the mutant with gene phaC1 from Pseudomonas sp. strain 61-3 in Escherichia coli does not confer the ability to synthesize polyhydroxyalkanoates, overview | Escherichia coli |
| F87P | site-directed mutagenesis, coexpression of the mutant enzyme with the phaC gene from Aeromonas caviae in a recombinant strain does not confer the ability to synthesize polyhydroxyalkanoates, coexpression of the mutant with gene phaC1 from Pseudomonas sp. strain 61-3 in Escherichia coli does not confer the ability to synthesize polyhydroxyalkanoates, overview | Escherichia coli |
| F87Q | site-directed mutagenesis, coexpression of the mutant enzyme with the phaC gene from Aeromonas caviae in a recombinant strain confers the ability to synthesize polyhydroxyalkanoates, coexpression of the mutant with gene phaC1 from Pseudomonas sp. strain 61-3 in Escherichia coli does not confer the ability to synthesize polyhydroxyalkanoates, overview | Escherichia coli |
| F87R | site-directed mutagenesis, coexpression of the mutant enzyme with the phaC gene from Aeromonas caviae in a recombinant strain does not confer the ability to synthesize polyhydroxyalkanoates, coexpression of the mutant with gene phaC1 from Pseudomonas sp. strain 61-3 in Escherichia coli does not confer the ability to synthesize polyhydroxyalkanoates, overview | Escherichia coli |
| F87S | site-directed mutagenesis, coexpression of the mutant enzyme with the phaC gene from Aeromonas caviae in a recombinant strain confers the ability to synthesize polyhydroxyalkanoates, coexpression of the mutant with gene phaC1 from Pseudomonas sp. strain 61-3 in Escherichia coli confers the ability to synthesize polyhydroxyalkanoates, overview | Escherichia coli |
| F87T | site-directed mutagenesis, coexpression of the mutant enzyme with the phaC gene from Aeromonas caviae in a recombinant strain confers the ability to synthesize polyhydroxyalkanoates, coexpression of the mutant with gene phaC1 from Pseudomonas sp. strain 61-3 in Escherichia coli confers the ability to synthesize polyhydroxyalkanoates, overview | Escherichia coli |
| F87V | site-directed mutagenesis, coexpression of the mutant enzyme with the phaC gene from Aeromonas caviae in a recombinant strain confers the ability to synthesize polyhydroxyalkanoates, coexpression of the mutant with gene phaC1 from Pseudomonas sp. strain 61-3 in Escherichia coli does not confer the ability to synthesize polyhydroxyalkanoates, overview | Escherichia coli |
| F87W | site-directed mutagenesis, coexpression of the mutant enzyme with the phaC gene from Aeromonas caviae in a recombinant strain confers the ability to synthesize polyhydroxyalkanoates, coexpression of the mutant with gene phaC1 from Pseudomonas sp. strain 61-3 in Escherichia coli confers the ability to synthesize polyhydroxyalkanoates, overview | Escherichia coli |
| F87Y | site-directed mutagenesis, coexpression of the mutant enzyme with the phaC gene from Aeromonas caviae in a recombinant strain confers the ability to synthesize polyhydroxyalkanoates, coexpression of the mutant with gene phaC1 from Pseudomonas sp. strain 61-3 in Escherichia coli confers the ability to synthesize polyhydroxyalkanoates, overview | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Escherichia coli | the wild-type enzyme is able to induce synthesis of polyhydroxyalkanoates PHA copolymers from glucose in a strain which is transformed with gene phaC from Aeromonas caviae or gene phaC1 from Pseudomonas sp. strain 61-3, thus fatty acid biosynthesis may supply monomers for PHA production, overview | ? | - |
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Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
gene fabH | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the wild-type enzyme is able to induce synthesis of polyhydroxyalkanoates PHA copolymers from glucose in a strain which is transformed with gene phaC from Aeromonas caviae or gene phaC1 from Pseudomonas sp. strain 61-3, thus fatty acid biosynthesis may supply monomers for PHA production, overview | Escherichia coli | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 3-ketoacyl-ACP synthase III | - |
Escherichia coli |
| FabH | - |
Escherichia coli |
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