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Literature summary for 2.3.1.180 extracted from
- Overexpression of 3-ketoacyl-acyl-carrier protein synthase IIIs in plants reduces the rate of lipid synthesis (2001), Plant Physiol., 125, 1103-1114.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of the enzyme in transgenic Nicotiana tabacum using the Agrobacterium tumefaciens transfection system | Spinacia oleracea |
| isozymes KAS III-1 and KAS III-2, DNA and amino acid sequence determination and analysis, seed-specific expression of the isozymes in transgenic Nicotiana tabacum, Brassica napus, and Arabidopsis thaliana using a napin gene promoter and the Agrobacterium tumefaciens transfection system | Cuphea hookeriana |
| seed-specific expression of the isozymes in transgenic Nicotiana tabacum, Brassica napus, and Arabidopsis thaliana using a napin gene promoter and the Agrobacterium tumefaciens transfection system | Brassica napus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | analysis of fatty acid composition and acyl-ACP content of transgenic plants expressing the two isozymes of Cuphea glaucescens, the transgenic plants show altered fatty acid levels with accumulation of palmitate | Cuphea hookeriana |
| additional information | transfection with Cuphea glaucescens isozymes KAS III-1 and KAS III-2 leads to altered fatty acid levels with accumulation of palmitate | Brassica napus |
| additional information | transfections with Cuphea glaucescens isozymes KAS III-1 and KAS III-2 or the spinach enzyme lead to altered fatty acid levels with accumulation of palmitate | Nicotiana tabacum |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42000 | - |
x * 42000, about, amino acid sequence calculation, isozyme KAS III-1, x * 43000, about, amino acid sequence calculation, isozyme KAS III-2 | Cuphea hookeriana |
| 43000 | - |
x * 42000, about, amino acid sequence calculation, isozyme KAS III-1, x * 43000, about, amino acid sequence calculation, isozyme KAS III-2 | Cuphea hookeriana |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + malonyl-[acyl-carrier protein] | Nicotiana tabacum | - |
acetoacetyl-[acyl-carrier protein] + CoA + CO2 | - |
? |  |
| acetyl-CoA + malonyl-[acyl-carrier protein] | Brassica napus | - |
acetoacetyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
| acetyl-CoA + malonyl-[acyl-carrier protein] | Cuphea hookeriana | - |
acetoacetyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
| acetyl-CoA + malonyl-[acyl-carrier protein] | Spinacia oleracea | - |
acetoacetyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
| additional information | Nicotiana tabacum | fatty acid composition and acyl-ACP content in the leaves | ? | - |
? | |
| additional information | Spinacia oleracea | fatty acid composition and acyl-ACP content in the leaves | ? | - |
? | |
| additional information | Brassica napus | fatty acid composition and acyl-ACP content in the seeds | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Brassica napus | - |
- |
- |
| Cuphea hookeriana | - |
two isozymes KAS III-1 and KAS III-2 | - |
| Nicotiana tabacum | - |
- |
- |
| Spinacia oleracea | Q07510 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| embryo | - |
Brassica napus | - |
| flower | constitutive expression of isozymes KAS III-1 and KAS III-2 | Cuphea hookeriana | - |
| leaf | - |
Nicotiana tabacum | - |
| leaf | - |
Spinacia oleracea | - |
| leaf | constitutive expression of isozymes KAS III-1 and KAS III-2 | Cuphea hookeriana | - |
| additional information | quantitatiev RT-PCR expression analysis of isozymes KAS III-1 and KAS III-2 | Cuphea hookeriana | - |
| root | constitutive expression of isozymes KAS III-1 and KAS III-2 | Cuphea hookeriana | - |
| seed | - |
Brassica napus | - |
| seed | developing, constitutive expression of isozymes KAS III-1 and KAS III-2 | Cuphea hookeriana | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
activity of wild-type embryo and those from transgenic plants | Brassica napus |
| 0.000106 | - |
wild-type leaves | Nicotiana tabacum |
| 0.0062 | - |
transgenic leaves transformed with the Cuphea glaucescens enzyme, average value | Nicotiana tabacum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + malonyl-[acyl-carrier protein] | - |
Nicotiana tabacum | acetoacetyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
| acetyl-CoA + malonyl-[acyl-carrier protein] | - |
Brassica napus | acetoacetyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
| acetyl-CoA + malonyl-[acyl-carrier protein] | - |
Cuphea hookeriana | acetoacetyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
| acetyl-CoA + malonyl-[acyl-carrier protein] | - |
Spinacia oleracea | acetoacetyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
| additional information | fatty acid composition and acyl-ACP content in the leaves | Nicotiana tabacum | ? | - |
? | |
| additional information | fatty acid composition and acyl-ACP content in the leaves | Spinacia oleracea | ? | - |
? | |
| additional information | fatty acid composition and acyl-ACP content in the seeds | Brassica napus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 42000, about, amino acid sequence calculation, isozyme KAS III-1, x * 43000, about, amino acid sequence calculation, isozyme KAS III-2 | Cuphea hookeriana |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 3-ketoacyl-acyl-carrier protein synthase III | - |
Nicotiana tabacum |
| 3-ketoacyl-acyl-carrier protein synthase III | - |
Brassica napus |
| 3-ketoacyl-acyl-carrier protein synthase III | - |
Cuphea hookeriana |
| 3-ketoacyl-acyl-carrier protein synthase III | - |
Spinacia oleracea |
| ACP synthase III | - |
Nicotiana tabacum |
| ACP synthase III | - |
Brassica napus |
| ACP synthase III | - |
Cuphea hookeriana |
| ACP synthase III | - |
Spinacia oleracea |
| KAS III | - |
Nicotiana tabacum |
| KAS III | - |
Brassica napus |
| KAS III | - |
Cuphea hookeriana |
| KAS III | - |
Spinacia oleracea |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Nicotiana tabacum |
| 30 | - |
assay at | Brassica napus |
| 30 | - |
assay at | Cuphea hookeriana |
| 30 | - |
assay at | Spinacia oleracea |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
assay at | Nicotiana tabacum |
| 6 | - |
assay at | Brassica napus |
| 6 | - |
assay at | Cuphea hookeriana |
| 6 | - |
assay at | Spinacia oleracea |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Cuphea hookeriana | isozyme KAS III-2, amino acid sequence calculation | - |
5.9 |
| Cuphea hookeriana | isozyme KAS III-1, amino acid sequence calculation | - |
6.8 |
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