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Literature summary for 2.3.1.180 extracted from

  • Matsumoto, K.; Murata, T.; Nagao, R.; Nomura, C.T.; Arai, S.; Arai, Y.; Takase, K.; Nakashita, H.; Taguchi, S.; Shimada, H.
    Production of short-chain-length/medium-chain-length polyhydroxyalkanoate (PHA) copolymer in the plastid of Arabidopsis thaliana using an engineered 3-ketoacyl-acyl carrier protein synthase III (2009), Biomacromolecules, 10, 686-690.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of mutant F87T in Arabidopsis thaliana plastids Escherichia coli

Protein Variants

Protein Variants Comment Organism
F87T coexpression of the enzyme in Arabidopsis thaliana plastids with Ser325Thr/Gln481Lys mutated polyhydroxyalkanoate synthase from Pseudomonas sp. 61-3, along with the beta-ketothiolase PhaA and acetoacetyl-CoA reductase PhaB from Ralstonia eutropha for successful production of short-chain-length/medium-chain-length polyhydroxyalkanoates in the transgenic plant plastids, expression of the engineered fabH gene in the plastid leads to an increase in the amount of the SCL monomer, 3-hydroxybutyrate, incorporated into polyhydroxyalkanoates, and contributes to supply of medium-chain-length monomers for polyhydroxyalkanoate production, overview Escherichia coli
additional information coexpression of the KASIII mutant F87T enzyme from Escherichia coli in Arabidopsis thaliana plastids together with Ser325Thr/Gln481Lys mutated polyhydroxyalkanoate synthase from Pseudomonas sp. 61-3, along with the beta-ketothiolase PhaA and acetoacetyl-CoA reductase PhaB from Ralstonia eutropha for successful production of short-chain-length/medium-chain-length polyhydroxyalkanoates in the transgenic plant plastids, expression of the engineered Escherichia coli fabH gene in the plastid leads to an increase in the amount of the SCL monomer, 3-hydroxybutyrate, incorporated into polyhydroxyalkanoates, and contributes to supply of medium-chain-length monomers for polyhydroxyalkanoate production, overview Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
Arabidopsis thaliana 9507
-

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-
Escherichia coli
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Arabidopsis thaliana
-

Synonyms

Synonyms Comment Organism
3-ketoacyl-ACP synthase III
-
Escherichia coli
3-ketoacyl-ACP synthase III
-
Arabidopsis thaliana
3-ketoacyl-acyl carrier protein synthase III
-
Escherichia coli
3-ketoacyl-acyl carrier protein synthase III
-
Arabidopsis thaliana
FabH
-
Escherichia coli
FabH
-
Arabidopsis thaliana

General Information

General Information Comment Organism
metabolism KASIII is involved in the biosynthesis of short-chain-length/medium-chain-length polyhydroxyalkanoate, pathway overview Arabidopsis thaliana