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Literature summary for 2.3.1.180 extracted from

  • Li, H.Q.; Luo, Y.; Zhu, H.L.
    Discovery of vinylogous carbamates as a novel class of beta-ketoacyl-acyl carrier protein synthase III (FabH) inhibitors (2011), Bioorg. Med. Chem., 19, 4454-4459.
    View publication on PubMed
Search for more literature for 2.3.1.180

Cloned(Commentary)

Cloned (Comment) Organism
overexpressed in E. coli DH10B cells using the pET30 vector, selenomethionine-substituted protein expressed in Escherichia coli BL21(DE3) Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
(E)-ethyl 2-(4-(benzyloxy)phenyl)-3-(4-bromophenylamino)acrylate
-
 Escherichia coli
(E)-ethyl 2-(4-(benzyloxy)phenyl)-3-(4-isopropylphenylamino)acrylate
-
 Escherichia coli
(Z)-ethyl 2-(4-(benzyloxy)phenyl)-3-(2,4-dibromophenylamino)acrylate
-
 Escherichia coli
(Z)-ethyl 2-(4-(benzyloxy)phenyl)-3-(2-chlorophenylamino)acrylate
-
 Escherichia coli
(Z)-ethyl 2-(4-(benzyloxy)phenyl)-3-(4-bromophenylamino)acrylate
-
 Escherichia coli
(Z)-ethyl 2-(4-(benzyloxy)phenyl)-3-(4-fluorophenylamino)acrylate
-
 Escherichia coli
(Z)-ethyl 3-(p-toluidino)-2-(4-(benzyloxy)phenyl)acrylate
-
 Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity in three chromatographic steps (Q-Sepharose, MonoQ, and hydroxyapatite), immobilized metal ion affinity chromatography (Ni2+) Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
malonyl-ACP + acetyl-CoA
-
 Escherichia coli CO2 + CoA + acetoacetyl-ACP
-
 ?

Synonyms

Synonyms Comment Organism
beta-ketoacyl-acyl carrier protein synthase III
-
 Escherichia coli
FabH
-
 Escherichia coli

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0026
-
 pH 7.0, temperature not specified in the publication Escherichia coli (Z)-ethyl 3-(p-toluidino)-2-(4-(benzyloxy)phenyl)acrylate
0.0033
-
 pH 7.0, temperature not specified in the publication Escherichia coli (Z)-ethyl 2-(4-(benzyloxy)phenyl)-3-(4-bromophenylamino)acrylate
0.0082
-
 pH 7.0, temperature not specified in the publication Escherichia coli (Z)-ethyl 2-(4-(benzyloxy)phenyl)-3-(4-fluorophenylamino)acrylate
0.0085
-
 pH 7.0, temperature not specified in the publication Escherichia coli (E)-ethyl 2-(4-(benzyloxy)phenyl)-3-(4-isopropylphenylamino)acrylate
0.024
-
 pH 7.0, temperature not specified in the publication Escherichia coli (Z)-ethyl 2-(4-(benzyloxy)phenyl)-3-(2,4-dibromophenylamino)acrylate
0.028
-
 pH 7.0, temperature not specified in the publication Escherichia coli (Z)-ethyl 2-(4-(benzyloxy)phenyl)-3-(2-chlorophenylamino)acrylate
0.043
-
 pH 7.0, temperature not specified in the publication Escherichia coli (E)-ethyl 2-(4-(benzyloxy)phenyl)-3-(4-bromophenylamino)acrylate