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Literature summary for 2.3.1.180 extracted from

  • Abugrain, M.E.; Brumsted, C.J.; Osborn, A.R.; Philmus, B.; Mahmud, T.
    A highly promiscuous beta-ketoacyl-ACP synthase (KAS) III-like protein is involved in pactamycin biosynthesis (2017), ACS Chem. Biol., 12, 362-366 .
    View publication on PubMed
Search for more literature for 2.3.1.180

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Streptomyces pactum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 10 mM used in assay conditions Streptomyces pactum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + a malonyl-[acyl-carrier protein] Streptomyces pactum
-
 an acetoacetyl-[acyl-carrier protein] + CoA + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces pactum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Streptomyces pactum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + a malonyl-[acyl-carrier protein]
-
 Streptomyces pactum an acetoacetyl-[acyl-carrier protein] + CoA + CO2
-
 ?
de-(6-methylsalicylyl)-7-deoxy-7-demethylpactamycin + S-(2-acetamidoethyl) (2E)-2,4-dimethylpent-2-enethioate 44% conversion Streptomyces pactum [(1S,2R,3S,4S,5S)-5-(3-acetylanilino)-4-amino-3-[(dimethylcarbamoyl)amino]-1,2-dihydroxy-2,3-dimethylcyclopentyl]methyl (3E)-3,5-dimethylhex-3-enoate + N-(2-sulfanylethyl)acetamide
-
 ?
de-(6-methylsalicylyl)-7-deoxy-7-demethylpactamycin + S-(2-acetamidoethyl) 2-hydroxy-6-methylbenzene-1-carbothioate 100% conversion Streptomyces pactum 7-deoxy-7-demethylpactamycin + N-(2-sulfanylethyl)acetamide
-
 ?
de-(6-methylsalicylyl)-7-deoxy-7-demethylpactamycin + S-(2-acetamidoethyl) 2-methylbutanethioate 20% conversion Streptomyces pactum [(1S,2R,3S,4S,5S)-5-(3-acetylanilino)-4-amino-3-[(dimethylcarbamoyl)amino]-1,2-dihydroxy-2,3-dimethylcyclopentyl]methyl 2-methylbutanoate + N-(2-sulfanylethyl)acetamide
-
 ?
de-(6-methylsalicylyl)-7-deoxy-7-demethylpactamycin + S-(2-acetamidoethyl) 2-methylpropanethioate 83% conversion Streptomyces pactum [(1S,2R,3S,4S,5S)-5-(3-acetylanilino)-4-amino-3-[(dimethylcarbamoyl)amino]-1,2-dihydroxy-2,3-dimethylcyclopentyl]methyl 2-methylpropanoate + N-(2-sulfanylethyl)acetamide
-
 ?
de-(6-methylsalicylyl)-7-deoxy-7-demethylpactamycin + S-(2-acetamidoethyl) 3-aminobenzene-1-carbothioate 17% conversion Streptomyces pactum [(1S,2R,3S,4S,5S)-5-(3-acetylanilino)-4-amino-3-[(dimethylcarbamoyl)amino]-1,2-dihydroxy-2,3-dimethylcyclopentyl]methyl 3-aminobenzoate + N-(2-sulfanylethyl)acetamide
-
 ?
de-(6-methylsalicylyl)-7-deoxy-7-demethylpactamycin + S-(2-acetamidoethyl) 3-methylbutanethioate 61% conversion Streptomyces pactum [(1S,2R,3S,4S,5S)-5-(3-acetylanilino)-4-amino-3-[(dimethylcarbamoyl)amino]-1,2-dihydroxy-2,3-dimethylcyclopentyl]methyl 3-methylbutanoate + N-(2-sulfanylethyl)acetamide
-
 ?
de-(6-methylsalicylyl)-7-deoxy-7-demethylpactamycin + S-(2-acetamidoethyl) 4-chlorobutanethioate 100% conversion Streptomyces pactum [(1S,2R,3S,4S,5S)-5-(3-acetylanilino)-4-amino-3-[(dimethylcarbamoyl)amino]-1,2-dihydroxy-2,3-dimethylcyclopentyl]methyl 4-chlorobutanoate + N-(2-sulfanylethyl)acetamide
-
 ?
de-(6-methylsalicylyl)-7-deoxy-7-demethylpactamycin + S-(2-acetamidoethyl) benzenecarbothioate 43% conversion Streptomyces pactum [(1S,2R,3S,4S,5S)-5-(3-acetylanilino)-4-amino-3-[(dimethylcarbamoyl)amino]-1,2-dihydroxy-2,3-dimethylcyclopentyl]methyl benzoate + N-(2-sulfanylethyl)acetamide
-
 ?
de-(6-methylsalicylyl)-7-deoxy-7-demethylpactamycin + S-(2-acetamidoethyl) butanethioate 91% conversion Streptomyces pactum [(1S,2R,3S,4S,5S)-5-(3-acetylanilino)-4-amino-3-[(dimethylcarbamoyl)amino]-1,2-dihydroxy-2,3-dimethylcyclopentyl]methyl butanoate + N-(2-sulfanylethyl)acetamide
-
 ?
de-(6-methylsalicylyl)-7-deoxy-7-demethylpactamycin + S-(2-acetamidoethyl) cycloheptanecarbothioate 87% conversion Streptomyces pactum [(1S,2R,3S,4S,5S)-5-(3-acetylanilino)-4-amino-3-[(dimethylcarbamoyl)amino]-1,2-dihydroxy-2,3-dimethylcyclopentyl]methyl cycloheptanecarboxylate + N-(2-sulfanylethyl)acetamide
-
 ?
de-(6-methylsalicylyl)-7-deoxy-7-demethylpactamycin + S-(2-acetamidoethyl) cyclohexanecarbothioate 57% conversion Streptomyces pactum [(1S,2R,3S,4S,5S)-5-(3-acetylanilino)-4-amino-3-[(dimethylcarbamoyl)amino]-1,2-dihydroxy-2,3-dimethylcyclopentyl]methyl cyclohexanecarboxylate + N-(2-sulfanylethyl)acetamide
-
 ?
de-(6-methylsalicylyl)-7-deoxy-7-demethylpactamycin + S-(2-acetamidoethyl) ethanethioate 71% conversion Streptomyces pactum [(1S,2R,3S,4S,5S)-5-(3-acetylanilino)-4-amino-3-[(dimethylcarbamoyl)amino]-1,2-dihydroxy-2,3-dimethylcyclopentyl]methyl acetate + N-(2-sulfanylethyl)acetamide
-
 ?
de-(6-methylsalicylyl)-7-deoxy-7-demethylpactamycin + S-(2-acetamidoethyl) phenylethanethioate 95% conversion Streptomyces pactum [(1S,2R,3S,4S,5S)-5-(3-acetylanilino)-4-amino-3-[(dimethylcarbamoyl)amino]-1,2-dihydroxy-2,3-dimethylcyclopentyl]methyl phenylacetate + N-(2-sulfanylethyl)acetamide
-
 ?
de-(6-methylsalicylyl)-7-deoxy-7-demethylpactamycin + S-(2-acetamidoethyl) propanethioate 87% conversion Streptomyces pactum [(1S,2R,3S,4S,5S)-5-(3-acetylanilino)-4-amino-3-[(dimethylcarbamoyl)amino]-1,2-dihydroxy-2,3-dimethylcyclopentyl]methyl propanoate + N-(2-sulfanylethyl)acetamide
-
 ?
de-6-methylsalicylyl-7-deoxypactamycin + 6-methylsalicylyl N-acetylcysteamine thioester
-
 Streptomyces pactum ?
-
 ?
de-6-methylsalicylyl-pactamycate + 6-methylsalicylyl N-acetylcysteamine thioester
-
 Streptomyces pactum ?
-
 ?
[PtmQ]-S-methyl 2-methylbenzene-1-carbothioate + de-6-methylsalicylyl-pactamycin
-
 Streptomyces pactum pactamycin + ?
-
 ?

Subunits

Subunits Comment Organism
? x * 39000, His6-tagged enzyme, SDS-PAGE Streptomyces pactum

Synonyms

Synonyms Comment Organism
beta-ketoacyl-ACP synthase III-like protein
-
 Streptomyces pactum
beta-ketoacyl-acyl carrier protein synthase III
-
 Streptomyces pactum
KAS III-like protein
-
 Streptomyces pactum
PtmR
-
 Streptomyces pactum

General Information

General Information Comment Organism
metabolism the enzyme is involved in pactamycin biosynthesis Streptomyces pactum