Cloned (Comment) | Organism |
---|---|
hexahistidine-tagged YpfI | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic parameters for ATP-hydrolysis or GTP-hydrolysis | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
[elongator tRNAMet]-cytidine34 + ATP + acetyl-CoA | Escherichia coli | tRNAMet cytidine acetyltransferase acetylates the wobble base C34 of the elongation-specific tRNAMet. TmcA specifically recognizes the anticodon stem of tRNAMet, thus distinguishing between tRNAMet and tRNAIle2, which is structurally similar and has the same anticodon loop | [elongator tRNAMet]-N4-acetylcytidine34 + ADP + phosphate + coenzyme A | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
[elongator tRNAMet]-cytidine34 + ATP + acetyl-CoA | tRNAMet cytidine acetyltransferase acetylates the wobble base C34 of the elongation-specific tRNAMet. TmcA specifically recognizes the anticodon stem of tRNAMet, thus distinguishing between tRNAMet and tRNAIle2, which is structurally similar and has the same anticodon loop | Escherichia coli | [elongator tRNAMet]-N4-acetylcytidine34 + ADP + phosphate + coenzyme A | - |
? | |
[elongator tRNAMet]-cytidine34 + ATP + acetyl-CoA | ATP/GTP hydrolysis by TmcA is stimulated in the presence of acetyl-CoA and tRNAMet. TmcA hydrolyses ATP or GTP between the beta- and gamma-phosphates, as shown by the detection of labelled ADP (or GDP). A mutation study reveals that Escherichia coli TmcA strictly discriminates elongator tRNAMet from the structurally similar tRNAIle by mainly recognizing the C27G43 pair in the anticodon stem | Escherichia coli | [elongator tRNAMet]-N4-acetylcytidine34 + ADP + phosphate + coenzyme A | - |
? | |
[elongator tRNAMet]-cytidine34 + GTP + acetyl-CoA | - |
Escherichia coli | [elongator tRNAMet]-N4-acetylcytidine34 + GDP + phosphate + coenzyme A | - |
? |
Synonyms | Comment | Organism |
---|---|---|
tmcA | - |
Escherichia coli |
ypfI | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic parameters for ATP-hydrolysis or GTP-hydrolysis | Escherichia coli |
General Information | Comment | Organism |
---|---|---|
malfunction | a tmcA deletion strain does not show a growth defect. Thus, this modification does not appear to be required for faithful translation in an otherwise wild-type strain. A tmcA/dusC double deletion strain has a severe cold-sensitive growth defect | Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic parameters for ATP-hydrolysis or GTP-hydrolysis | Escherichia coli |