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Literature summary for 2.3.1.194 extracted from

  • Matsumoto, K.; Yamada, M.; Leong, C.; Jo, S.; Kuzuyama, T.; Taguchi, S.
    A new pathway for poly(3-hydroxybutyrate) production in Escherichia coli and Corynebacterium glutamicum by functional expression of a new acetoacetyl-coenzyme A synthase (2011), Biosci. Biotechnol. Biochem., 75, 364-366.
    View publication on PubMed
Search for more literature for 2.3.1.194

Application

Application Comment Organism
synthesis usage of the enzyme for recombinant high-level production of poly(3-hydroxybutyrate), P(3HB), in Escherichia coli and Corynebacterium glutamicum. P(3HB) is the natural aliphatic polyester that can be processed into a wide variety of consumer products, including plastics, films and fibers Streptomyces sp.

Cloned(Commentary)

Cloned (Comment) Organism
co-expression of polyhydroxyalkanoate synthase, PhaC, from Ralstonia eutropha with acetoacetyl-CoA synthetase in Escherichia coli and Corynebacterium glutamicum leading to enhanced production of polyhydroxybutanoates, by cloning the AACS gene into the phaABC operon of Ralstonia eutropha. Overexpression of AACS leads to a great enhancement of the malonyl-CoA pool in Escherichia coli Streptomyces sp.
recombinant expression of AACS in Corynebacterium glutamicum and Escherichia coli leads to aquired ability to form poly(3-hydroxybutyrate) in the transformed organisms by 19.7 wt% and 10.5 wt%, respectively, biosynthetic pathway, overview Streptomyces sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + malonyl-CoA Streptomyces sp.
-
 acetoacetyl-CoA + CoA + CO2
-
 ?
acetyl-CoA + malonyl-CoA Streptomyces sp.
-
 CoA + acetoacetyl-CoA + CO2
-
 ir

Organism

Organism UniProt Comment Textmining
Streptomyces sp.
-
-
-
Streptomyces sp.
-
 gene nphT7
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + malonyl-CoA
-
 Streptomyces sp. acetoacetyl-CoA + CoA + CO2
-
 ?
acetyl-CoA + malonyl-CoA
-
 Streptomyces sp. CoA + acetoacetyl-CoA + CO2
-
 ir

Synonyms

Synonyms Comment Organism
AACS
-
 Streptomyces sp.
Acetoacetyl-CoA synthase
-
 Streptomyces sp.
Acetoacetyl-CoA synthetase
-
 Streptomyces sp.
acetoacetyl-coenzyme A synthase
-
 Streptomyces sp.
More the enzyme belongs to the thiolase superfamily of enzymes Streptomyces sp.
NphT7
-
 Streptomyces sp.

General Information

General Information Comment Organism
metabolism polyhydroxyalkanoate synthase, PhaC, catalyzes generation of (R)-3-hydroxybutyrate monomers from two acetyl-CoA molecules, and further of short-chain length polyhydroxyalkanoates, PHAs. The malonyl-CoA availability is a limiting factor to synthesis of poly(3-hydroxybutyrate), P(3HB), thus acetoacetyl-CoA synthetase, which is controlling the malonyl-CoA pool, is an important enzyme for increasing the P(3HB) production Streptomyces sp.
additional information alterations in acety-CoA carboxylase activity to optimize the acetyl-CoA/malonyl-CoA ratio might be an effective way to increase poly(3-hydroxybutyrate) production via the AACS-catalyzed paythway Streptomyces sp.