Cloned (Comment) | Organism |
---|---|
gene AK109558, expression of His6-tagged CUS in Escherichia coli strain BL21(DE3)-pLysS | Oryza sativa |
Crystallization (Comment) | Organism |
---|---|
purified recombinant His6-tagged enzyme, sitting drop vapour diffusion method, optimization of the crystallization conditions, mixing 0.0005 ml of protein solution, containing 10 mg/ml in 20 mM HEPES-NaOH, pH 7.0, 100 mM NaCl, and 2 mM DTT, with an equal volume of reservoir solution, containing 100 mM HEPES-NaOH, pH 7.8, 1995 mM ammonium sulfate, 3% dimethylsulfoxide, and 2 mM CoA, and equilibration against 0.1 ml reservoir solution, 20°C, cryoprotection in 100 mM HEPES-NaOH pH 7.8, 1330 mM ammonium sulfate, 2 mM CoA, 12% v/v glycerol and 14% w/v PEG 400, X-ray diffraction structure determination and analysis st 2.5 A resolution | Oryza sativa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 4-coumaroyl-CoA + malonyl-CoA + H2O | Oryza sativa | - |
3 CoA + bisdemethoxycurcumin + 2 CO2 | - |
? | |
2 feruloyl-CoA + malonyl-CoA + H2O | Oryza sativa | - |
3 CoA + curcumin + 2 CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa | - |
gene AK109558 | - |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3)-pLysS by nickel affinity and anion exchange chromatography, followed by gel filtration | Oryza sativa |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2 4-coumaroyl-CoA + malonyl-CoA + H2O = 3 CoA + bisdemethoxycurcumin + 2 CO2 | the reaction proceeds in three steps via formation of 4-coumaroyldiketide-CoA and 4-coumaroyl beta-keto acid, overview | Oryza sativa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 4-coumaroyl-CoA + malonyl-CoA + H2O | - |
Oryza sativa | 3 CoA + bisdemethoxycurcumin + 2 CO2 | - |
? | |
2 4-coumaroyl-CoA + malonyl-CoA + H2O | the reaction proceeds in three steps via formation of 4-coumaroyldiketide-CoA and 4-coumaroyl beta-keto acid, overview | Oryza sativa | 3 CoA + bisdemethoxycurcumin + 2 CO2 | - |
? | |
2 feruloyl-CoA + malonyl-CoA + H2O | - |
Oryza sativa | 3 CoA + curcumin + 2 CO2 | - |
? | |
2 feruloyl-CoA + malonyl-CoA + H2O | the reaction proceeds in three steps via formation of feruloyldiketide-CoA and feruloyl beta-keto acid, overview | Oryza sativa | 3 CoA + curcumin + 2 CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | enzyme structure-function relationship, overview | Oryza sativa |
Synonyms | Comment | Organism |
---|---|---|
curcuminoid synthase | - |
Oryza sativa |
CUS | - |
Oryza sativa |
General Information | Comment | Organism |
---|---|---|
evolution | curcuminoid synthase is a plant-specific type III polyketide synthase and belongs to the chalcone synthase superfamily of type III polyketide synthases | Oryza sativa |
additional information | enzyme structure-function relationship, overview | Oryza sativa |