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Literature summary for 2.3.1.211 extracted from

  • Morita, H.; Wanibuchi, K.; Kato, R.; Sugio, S.; Abe, I.
    Expression, purification and crystallization of a plant type III polyketide synthase that produces diarylheptanoids (2010), Acta Crystallogr. Sect. F, 66, 948-950.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene AK109558, expression of His6-tagged CUS in Escherichia coli strain BL21(DE3)-pLysS Oryza sativa

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant His6-tagged enzyme, sitting drop vapour diffusion method, optimization of the crystallization conditions, mixing 0.0005 ml of protein solution, containing 10 mg/ml in 20 mM HEPES-NaOH, pH 7.0, 100 mM NaCl, and 2 mM DTT, with an equal volume of reservoir solution, containing 100 mM HEPES-NaOH, pH 7.8, 1995 mM ammonium sulfate, 3% dimethylsulfoxide, and 2 mM CoA, and equilibration against 0.1 ml reservoir solution, 20°C, cryoprotection in 100 mM HEPES-NaOH pH 7.8, 1330 mM ammonium sulfate, 2 mM CoA, 12% v/v glycerol and 14% w/v PEG 400, X-ray diffraction structure determination and analysis st 2.5 A resolution Oryza sativa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 4-coumaroyl-CoA + malonyl-CoA + H2O Oryza sativa
-
3 CoA + bisdemethoxycurcumin + 2 CO2
-
?
2 feruloyl-CoA + malonyl-CoA + H2O Oryza sativa
-
3 CoA + curcumin + 2 CO2
-
?

Organism

Organism UniProt Comment Textmining
Oryza sativa
-
gene AK109558
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3)-pLysS by nickel affinity and anion exchange chromatography, followed by gel filtration Oryza sativa

Reaction

Reaction Comment Organism Reaction ID
2 4-coumaroyl-CoA + malonyl-CoA + H2O = 3 CoA + bisdemethoxycurcumin + 2 CO2 the reaction proceeds in three steps via formation of 4-coumaroyldiketide-CoA and 4-coumaroyl beta-keto acid, overview Oryza sativa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 4-coumaroyl-CoA + malonyl-CoA + H2O
-
Oryza sativa 3 CoA + bisdemethoxycurcumin + 2 CO2
-
?
2 4-coumaroyl-CoA + malonyl-CoA + H2O the reaction proceeds in three steps via formation of 4-coumaroyldiketide-CoA and 4-coumaroyl beta-keto acid, overview Oryza sativa 3 CoA + bisdemethoxycurcumin + 2 CO2
-
?
2 feruloyl-CoA + malonyl-CoA + H2O
-
Oryza sativa 3 CoA + curcumin + 2 CO2
-
?
2 feruloyl-CoA + malonyl-CoA + H2O the reaction proceeds in three steps via formation of feruloyldiketide-CoA and feruloyl beta-keto acid, overview Oryza sativa 3 CoA + curcumin + 2 CO2
-
?

Subunits

Subunits Comment Organism
More enzyme structure-function relationship, overview Oryza sativa

Synonyms

Synonyms Comment Organism
curcuminoid synthase
-
Oryza sativa
CUS
-
Oryza sativa

General Information

General Information Comment Organism
evolution curcuminoid synthase is a plant-specific type III polyketide synthase and belongs to the chalcone synthase superfamily of type III polyketide synthases Oryza sativa
additional information enzyme structure-function relationship, overview Oryza sativa