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Literature summary for 2.3.1.225 extracted from

  • Dunphy, J. T.; Greentree, W. K.; Linder, M. E.
    Enrichment of G-protein palmitoyltransferase activity in low density membranes (2001), J. Biol. Chem., 276, 43300-43304.
    View publication on PubMed
Search for more literature for 2.3.1.225

Inhibitors

Inhibitors Comment Organism Structure
additional information depletion of cellular cholesterol with the drug methyl-beta-cyclodextrin results in inhibition of palmitoyltransferase activity and a redistribution of the remaining activity to membranes of higher density, the process is reversible by cholesterol addition Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane PAT activity is highly enriched in low density membranes dependent upon cholesterol Bos taurus 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
palmitoyl-CoA + [G-protein alpha subunit Galphai]-L-cysteine Bos taurus
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 [G-protein alpha subunit Galphai]-S-palmitoyl-L-cysteine + CoA
-
 r
palmitoyl-CoA + [protein]-L-cysteine Bos taurus
-
 [protein]-S-palmitoyl-L-cysteine + CoA
-
 r

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
palmitoyl-CoA + [G-protein alpha subunit Galphai]-L-cysteine
-
 Bos taurus [G-protein alpha subunit Galphai]-S-palmitoyl-L-cysteine + CoA
-
 r
palmitoyl-CoA + [protein]-L-cysteine
-
 Bos taurus [protein]-S-palmitoyl-L-cysteine + CoA
-
 r

Synonyms

Synonyms Comment Organism
G-protein palmitoyltransferase
-
 Bos taurus
Pat
-
 Bos taurus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Bos taurus

General Information

General Information Comment Organism
malfunction depletion of cellular cholesterol with the drug methyl-beta-cyclodextrin results in inhibition of palmitoyltransferase activity and a redistribution of the remaining activity to membranes of higher density, the process is reversible by cholesterol addition Bos taurus
physiological function palmitoyltransferase facilitates the enrichment of fatty acylated signaling molecules in plasma membrane subdomains. Fatty acylation, involving the enzyme, is one mechanism for targeting proteins to lipid rafts, low density, sphingomyelin- and cholesterol-enriched membranes. When reconstituted into cell membranes, the population of purified recombinant Gi that is palmitoylated is highly enriched in the low density membrane fractions, whereas the bulk unmodified Gi-protein is largely excluded, the effect requires palmitoyltransferase activity and is abolished if the palmitoylated cysteine was mutated. Bos taurus