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Literature summary for 2.3.1.225 extracted from

  • Zeidman, R.; Buckland, G.; Cebecauer, M.; Eissmann, P.; Davis, D.M.; Magee, A.I.
    DHHC2 is a protein S-acyltransferase for Lck (2011), Mol. Membr. Biol., 28, 473-486.
    View publication on PubMed
Search for more literature for 2.3.1.225

Cloned(Commentary)

Cloned (Comment) Organism
DHHC16, cloning of cDNA from Jurkat cells, insertion into pmCFP via pENTR/D-TOPO, and expression in T cells deficient in Lck, the T lymphocyte-specific Src family kinase, transient expression of cyan fluorescent protein CFP-tagged DHHC16 in HEK293Acells Homo sapiens
DHHC2, cloning of cDNA from Jurkat cells, insertion into pmCFP via pENTR/D-TOPO, and expression in T cells deficient in Lck, the T lymphocyte-specific Src family kinase, transient expression of cyan fluorescent protein CFP-tagged DHHC2 in HEK293Acells Homo sapiens
DHHC4, cloning of cDNA from Jurkat cells, insertion into pmCFP via pENTR/D-TOPO, and expression in T cells deficient in Lck, the T lymphocyte-specific Src family kinase, transient expression of cyan fluorescent protein CFP-tagged DHHC4 in HEK293A cells, quantitative expression analysis Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information DHHC2 targeting with siRNA leads to Lck partial delocalization from the membranes and moving to the cytosol Homo sapiens
additional information DHHC4 targeting with siRNA leads to Lck partial delocalization from the membranes and moving to the cytosol Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum DHHC2 is present in at least some areas of the endoplasmic reticulum and Golgi Homo sapiens 5783
-
Golgi membrane DHHC2 is present in at least some areas of the endoplasmic reticulum and Golgi Homo sapiens 139
-
membrane associated Homo sapiens 16020
-
additional information the DHHC protein is not associated with early endosomes Homo sapiens
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
palmitoyl-CoA + [protein]-L-cysteine Homo sapiens
-
 [protein]-S-palmitoyl-L-cysteine + CoA
-
 r

Organism

Organism UniProt Comment Textmining
Homo sapiens Q969W1 ZDHC16, gene ZDHHC116; gene ZDHHC2
-
Homo sapiens Q9NPG8 ZDHC4, gene ZDHHC4; gene ZDHHC4
-
Homo sapiens Q9UIJ5 ZDHC2, gene ZDHHC2; gene ZDHHC2
-

Source Tissue

Source Tissue Comment Organism Textmining
JURKAT cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
palmitoyl-CoA + [protein]-L-cysteine
-
 Homo sapiens [protein]-S-palmitoyl-L-cysteine + CoA
-
 r

Synonyms

Synonyms Comment Organism
DHHC16
-
 Homo sapiens
DHHC2
-
 Homo sapiens
DHHC4
-
 Homo sapiens
Pat
-
 Homo sapiens

General Information

General Information Comment Organism
additional information overexpression of DHHC2 causes increased S-acylation of LckN10-GFP. In resting Jurkat T cells, endogenous DHHC2 and Lck, a non-receptor tyrosine kinase of the Src family, are in close proximity to each other at the cell periphery, but completely non-overlapping, DHHC2 is a PAT for Lck in vivo Homo sapiens
additional information overexpression of DHHC4 does not cause increased S-acylation of LckN10-GFP Homo sapiens