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Literature summary for 2.3.1.23 extracted from

  • Hasegawa-Sasaki, H.; Ohno, K.
    Extraction and partial purification of acyl-CoA:1-acyl-sn-glycero-3-phosphocholine acyltransferase from rat liver microsomes (1980), Biochim. Biophys. Acta, 617, 205-217.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
methanol activation, arachidonoyl-CoA as substrate Rattus norvegicus

General Stability

General Stability Organism
glycerol, 50%, stabilizes Rattus norvegicus
low ionic strength stabilizes during purification Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
Brij 35 0.1% Rattus norvegicus
Brij 98 0.1% Rattus norvegicus
Lubrol WX 0.1% Rattus norvegicus
methanol oleoyl-CoA as substrate Rattus norvegicus
additional information not inhibited by IAA and DTNB Rattus norvegicus
N-ethylmaleimide 1 mM, 30% inhibition after 5 min, 95% inhibition after 45 min Rattus norvegicus
p-chloromercuribenzoate partially restorable by dithiothreitol Rattus norvegicus
p-chloromercuriphenyl sulfonic acid partially restorable by dithitothreitol Rattus norvegicus
Triton X-100 0.1% Rattus norvegicus
Tween 20 0.1% Rattus norvegicus
Tween 80 0.1% Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0027
-
palmitoyl-CoA
-
Rattus norvegicus
0.0028
-
oleoyl-CoA
-
Rattus norvegicus
0.0032
-
Arachidonoyl-CoA
-
Rattus norvegicus
0.0035
-
linoleoyl-CoA
-
Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
Rattus norvegicus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
225000
-
gel filtration Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
male Wistar
-

Purification (Commentary)

Purification (Comment) Organism
partially purified Rattus norvegicus
Ultrogel AcA 34 Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3
-
acyl donor arachidonoyl-CoA Rattus norvegicus

Storage Stability

Storage Stability Organism
-20°C, multiple freeze-thawing cycles, 6 weeks, 20% loss of activity Rattus norvegicus
-20°C, partially purified enzyme, several months Rattus norvegicus
-20°C, purified enzyme, at least 3 weeks Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
-
Rattus norvegicus CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
r
arachidonoyl-CoA + 1-acyl-sn-glycero-3-phosphoethanolamine
-
Rattus norvegicus CoA + 1-acyl-2-arachidonoyl-sn-glycero-3-phosphoethanolamine
-
?
arachidonoyl-CoA + 1-acyl-sn-glycero-3-phosphoinositol
-
Rattus norvegicus CoA + 1-acyl-2-arachidonoyl-sn-glycero-3-phosphoinositol
-
?
arachidonoyl-CoA + 1-acyl-sn-glycero-3-phosphoserine
-
Rattus norvegicus CoA + 1-acyl-2-arachidonoyl-sn-glycero-3-phosphoserine
-
?
linoleoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
-
Rattus norvegicus CoA + 1-acyl-2-linoleoyl-sn-glycero-3-phosphocholine
-
?
oleoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
-
Rattus norvegicus CoA + 1-acyl-2-oleoyl-sn-glycero-3-phosphocholine
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Rattus norvegicus
30
-
-
Rattus norvegicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
oleoyl-CoA specific activity loses 50% of activity after 90 min, arachidonoyl-CoA specific activity loses 45% of activity after 90 min Rattus norvegicus
45
-
10% loss of activity after 1 min Rattus norvegicus
48
-
half-life: 1 min, oleoyl-CoA specific activity Rattus norvegicus
50
-
50% loss of activity after 1 min Rattus norvegicus
52
-
half-life: 1 min, arachidonoyl-CoA specific activity Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Rattus norvegicus

pH Range

pH Minimum pH Maximum Comment Organism
6 9 approx. half-maximal activity at pH 6.0 and 9.6 Rattus norvegicus