Activating Compound | Comment | Organism | Structure |
---|---|---|---|
methanol | activation, arachidonoyl-CoA as substrate | Rattus norvegicus |
General Stability | Organism |
---|---|
glycerol, 50%, stabilizes | Rattus norvegicus |
low ionic strength stabilizes during purification | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Brij 35 | 0.1% | Rattus norvegicus | |
Brij 98 | 0.1% | Rattus norvegicus | |
Lubrol WX | 0.1% | Rattus norvegicus | |
methanol | oleoyl-CoA as substrate | Rattus norvegicus | |
additional information | not inhibited by IAA and DTNB | Rattus norvegicus | |
N-ethylmaleimide | 1 mM, 30% inhibition after 5 min, 95% inhibition after 45 min | Rattus norvegicus | |
p-chloromercuribenzoate | partially restorable by dithiothreitol | Rattus norvegicus | |
p-chloromercuriphenyl sulfonic acid | partially restorable by dithitothreitol | Rattus norvegicus | |
Triton X-100 | 0.1% | Rattus norvegicus | |
Tween 20 | 0.1% | Rattus norvegicus | |
Tween 80 | 0.1% | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0027 | - |
palmitoyl-CoA | - |
Rattus norvegicus | |
0.0028 | - |
oleoyl-CoA | - |
Rattus norvegicus | |
0.0032 | - |
Arachidonoyl-CoA | - |
Rattus norvegicus | |
0.0035 | - |
linoleoyl-CoA | - |
Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Rattus norvegicus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
225000 | - |
gel filtration | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
male Wistar | - |
Purification (Comment) | Organism |
---|---|
partially purified | Rattus norvegicus |
Ultrogel AcA 34 | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.3 | - |
acyl donor arachidonoyl-CoA | Rattus norvegicus |
Storage Stability | Organism |
---|---|
-20°C, multiple freeze-thawing cycles, 6 weeks, 20% loss of activity | Rattus norvegicus |
-20°C, partially purified enzyme, several months | Rattus norvegicus |
-20°C, purified enzyme, at least 3 weeks | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine | - |
Rattus norvegicus | CoA + 1,2-diacyl-sn-glycero-3-phosphocholine | - |
r | |
arachidonoyl-CoA + 1-acyl-sn-glycero-3-phosphoethanolamine | - |
Rattus norvegicus | CoA + 1-acyl-2-arachidonoyl-sn-glycero-3-phosphoethanolamine | - |
? | |
arachidonoyl-CoA + 1-acyl-sn-glycero-3-phosphoinositol | - |
Rattus norvegicus | CoA + 1-acyl-2-arachidonoyl-sn-glycero-3-phosphoinositol | - |
? | |
arachidonoyl-CoA + 1-acyl-sn-glycero-3-phosphoserine | - |
Rattus norvegicus | CoA + 1-acyl-2-arachidonoyl-sn-glycero-3-phosphoserine | - |
? | |
linoleoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine | - |
Rattus norvegicus | CoA + 1-acyl-2-linoleoyl-sn-glycero-3-phosphocholine | - |
? | |
oleoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine | - |
Rattus norvegicus | CoA + 1-acyl-2-oleoyl-sn-glycero-3-phosphocholine | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Rattus norvegicus |
30 | - |
- |
Rattus norvegicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
oleoyl-CoA specific activity loses 50% of activity after 90 min, arachidonoyl-CoA specific activity loses 45% of activity after 90 min | Rattus norvegicus |
45 | - |
10% loss of activity after 1 min | Rattus norvegicus |
48 | - |
half-life: 1 min, oleoyl-CoA specific activity | Rattus norvegicus |
50 | - |
50% loss of activity after 1 min | Rattus norvegicus |
52 | - |
half-life: 1 min, arachidonoyl-CoA specific activity | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | approx. half-maximal activity at pH 6.0 and 9.6 | Rattus norvegicus |