Cloned (Comment) | Organism |
---|---|
expression in A-431 cell and COS-7 cell | Homo sapiens |
expression in A-431 cell, COS-7 cell, and Hu-H7 cell | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | - |
Homo sapiens | 5783 | - |
lipid droplet | in addition to endoplasmic reticulum, both isoforms LCAT1 and LCAT2 localize to lipid droplets, which consist of a core of neutral lipids surrounded by a monolayer of phospholipid, mainly phosphatidylcholine. This dual localization is enabled by the monotopic topology of the enzymes with both termini facing the cytoplasm, irrespective of supplementation with oleic acid. Lipid droplets have the ability to locally synthesize phosphatidylcholine and this activity correlates with the LPCAT1 and -2 expression levels | Homo sapiens | 5811 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q7L5N7 | isoform PCAT2 | - |
Homo sapiens | Q8NF37 | isoform PCAT1 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
A-431 cell | high expression of both isoforms LCAT1 and LCAT2 | Homo sapiens | - |
Huh-7 cell | low expression of isoform LCAT1 | Homo sapiens | - |
lung | - |
Homo sapiens | - |
Synonyms | Comment | Organism |
---|---|---|
PCAT1 | - |
Homo sapiens |
PCAT2 | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | double knockdown of both isoforms LPCAT1 and LPCAT2 in A-431 cells leads to strong reduction of lipid-droplet-associated enzymic activity | Homo sapiens |
physiological function | double knockdown of both isoforms LPCAT1 and LPCAT2 in A-431 cells or single knockdown of isoform LPCAT1 in Hu-H7 cells leads to strong reduction of lipid-droplet-associated enzymic activity. Reduction of LPCAT1 activity in Hu-H7 cells is associated with a morphological alteration in the lipid droplet pool, the mean lipid droplet size increases, although the amount of stored triacylglycerols remains constant | Homo sapiens |