BRENDA - Enzyme Database
show all sequences of 2.3.1.266

Crystal structure of RimI from Salmonella typhimurium LT2, the GNAT responsible for Nalpha-acetylation of ribosomal protein S18

Vetting, M.; Bareich, D.; Yu, M.; Blanchard, J.; Protein Sci. 17, 1781-1790 (2008) View publication on PubMedView publication on EuropePMC

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expressed in Escherichia coli Rosetta2 (DE3) pLysS cells
Salmonella enterica subsp. enterica serovar Typhimurium
Crystallization (Commentary)
Crystallization (Commentary)
Organism
in complex with CoA, acetyl-CoA, and a CoA-S-acetyl-ARYFRR bisubstrate inhibitor, vapor diffusion method, using
Salmonella enterica subsp. enterica serovar Typhimurium
Inhibitors
Inhibitors
Commentary
Organism
Structure
CoA-S-acetyl-ARYFRR
linearly competitive against acetyl-CoA and linearly noncompetitive against S18 protein(1-6)
Salmonella enterica subsp. enterica serovar Typhimurium
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
acetyl-CoA + an N-terminal L-alanyl-[S18 protein of 30S ribosome]
Salmonella enterica subsp. enterica serovar Typhimurium
-
CoA + an N-terminal N-acetyl-L-alanyl-[S18 protein of 30S ribosome]
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Salmonella enterica subsp. enterica serovar Typhimurium
Q8ZJW4
-
-
Purification (Commentary)
Purification (Commentary)
Organism
Ni HiTrap column chromatography
Salmonella enterica subsp. enterica serovar Typhimurium
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
acetyl-CoA + an N-terminal L-alanyl-[S18 protein of 30S ribosome]
-
746391
Salmonella enterica subsp. enterica serovar Typhimurium
CoA + an N-terminal N-acetyl-L-alanyl-[S18 protein of 30S ribosome]
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
RimI
-
Salmonella enterica subsp. enterica serovar Typhimurium
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.0041
-
CoA-S-acetyl-ARYFRR
at pH 7.3 and 25°C
Salmonella enterica subsp. enterica serovar Typhimurium
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli Rosetta2 (DE3) pLysS cells
Salmonella enterica subsp. enterica serovar Typhimurium
Crystallization (Commentary) (protein specific)
Crystallization
Organism
in complex with CoA, acetyl-CoA, and a CoA-S-acetyl-ARYFRR bisubstrate inhibitor, vapor diffusion method, using
Salmonella enterica subsp. enterica serovar Typhimurium
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
CoA-S-acetyl-ARYFRR
linearly competitive against acetyl-CoA and linearly noncompetitive against S18 protein(1-6)
Salmonella enterica subsp. enterica serovar Typhimurium
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.0041
-
CoA-S-acetyl-ARYFRR
at pH 7.3 and 25°C
Salmonella enterica subsp. enterica serovar Typhimurium
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
acetyl-CoA + an N-terminal L-alanyl-[S18 protein of 30S ribosome]
Salmonella enterica subsp. enterica serovar Typhimurium
-
CoA + an N-terminal N-acetyl-L-alanyl-[S18 protein of 30S ribosome]
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
Ni HiTrap column chromatography
Salmonella enterica subsp. enterica serovar Typhimurium
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
acetyl-CoA + an N-terminal L-alanyl-[S18 protein of 30S ribosome]
-
746391
Salmonella enterica subsp. enterica serovar Typhimurium
CoA + an N-terminal N-acetyl-L-alanyl-[S18 protein of 30S ribosome]
-
-
-
?
Other publictions for EC 2.3.1.266
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
745216
Kazakov
The RimL transacetylase provi ...
Escherichia coli
J. Bacteriol.
196
3377-3385
2014
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1
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1
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1
1
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746391
Vetting
Crystal structure of RimI fro ...
Salmonella enterica subsp. enterica serovar Typhimurium
Protein Sci.
17
1781-1790
2008
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1
1
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1
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1
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1
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1
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1
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1
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1
1
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1
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1
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1
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745256
Tercero
MAK3 encodes an N-acetyltrans ...
Saccharomyces cerevisiae
J. Biol. Chem.
267
20277-20281
1992
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-
-
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-
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1
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1
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1
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1
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1
1
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-
745779
Yoshikawa
Cloning and nucleotide sequen ...
Escherichia coli
Mol. Gen. Genet.
209
481-488
1987
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1
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1
2
1
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1
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1
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1
2
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745777
Isono
-
Ribosomal protein modificatio ...
Escherichia coli
Mol. Gen. Genet.
177
645-651
1980
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1
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