Crystal structure of RimI from Salmonella typhimurium LT2, the GNAT responsible for Nalpha-acetylation of ribosomal protein S18

Vetting, M.; Bareich, D.; Yu, M.; Blanchard, J.; Protein Sci. 17, 1781-1790 (2008)

Data extracted from this reference:

Inhibitors

Commentary

Organism

Structure

CoA-S-acetyl-ARYFRR

linearly competitive against acetyl-CoA and linearly noncompetitive against S18 protein(1-6)

Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates

Organism

Commentary (Nat. Sub.)

Natural Products

Commentary (Nat. Pro.)

Organism (Nat. Pro.)

Reversibility

ID

acetyl-CoA + an N-terminal L-alanyl-[S18 protein of 30S ribosome]

Salmonella enterica subsp. enterica serovar Typhimurium

-

CoA + an N-terminal N-acetyl-L-alanyl-[S18 protein of 30S ribosome]

-

-

?

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

Substrate Product ID

acetyl-CoA + an N-terminal L-alanyl-[S18 protein of 30S ribosome]

-

746391

Salmonella enterica subsp. enterica serovar Typhimurium

CoA + an N-terminal N-acetyl-L-alanyl-[S18 protein of 30S ribosome]

-

-

-

?

Ki Value [mM]

Ki Value maximum [mM]

Inhibitor

Commentary

Organism

Structure

0.0041

-

CoA-S-acetyl-ARYFRR

at pH 7.3 and 25°C

Salmonella enterica subsp. enterica serovar Typhimurium

Inhibitors

Commentary

Organism

Structure

CoA-S-acetyl-ARYFRR

linearly competitive against acetyl-CoA and linearly noncompetitive against S18 protein(1-6)

Salmonella enterica subsp. enterica serovar Typhimurium

Ki Value [mM]

Ki Value maximum [mM]

Inhibitor

Commentary

Organism

Structure

0.0041

-

CoA-S-acetyl-ARYFRR

at pH 7.3 and 25°C

Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates

Organism

Commentary (Nat. Sub.)

Natural Products

Commentary (Nat. Pro.)

Organism (Nat. Pro.)

Reversibility

ID

acetyl-CoA + an N-terminal L-alanyl-[S18 protein of 30S ribosome]

Salmonella enterica subsp. enterica serovar Typhimurium

-

CoA + an N-terminal N-acetyl-L-alanyl-[S18 protein of 30S ribosome]

-

-

?

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

ID

acetyl-CoA + an N-terminal L-alanyl-[S18 protein of 30S ribosome]

-

746391

Salmonella enterica subsp. enterica serovar Typhimurium

CoA + an N-terminal N-acetyl-L-alanyl-[S18 protein of 30S ribosome]

-

-

-

?

No.

1st author

Pub Med

title

organims

journal

volume

pages

year

Activating Compound

Application

Cloned(Commentary)

Crystallization (Commentary)

Engineering

General Stability

Inhibitors

KM Value [mM]

Localization

Metals/Ions

Molecular Weight [Da]

Natural Substrates/ Products (Substrates)

Organic Solvent Stability

Organism

Oxidation Stability

Posttranslational Modification

Purification (Commentary)

Reaction

Renatured (Commentary)

Source Tissue

Specific Activity [micromol/min/mg]

Storage Stability

Substrates and Products (Substrate)

Subunits

Synonyms

Temperature Optimum [°C]

Temperature Range [°C]

Temperature Stability [°C]

Turnover Number [1/s]

pH Optimum

pH Range

pH Stability

Cofactor

Ki Value [mM]

pI Value

IC50 Value

Activating Compound (protein specific)

Application (protein specific)

Cloned(Commentary) (protein specific)

Cofactor (protein specific)

Crystallization (Commentary) (protein specific)

Engineering (protein specific)

General Stability (protein specific)

IC50 Value (protein specific)

Inhibitors (protein specific)

Ki Value [mM] (protein specific)

KM Value [mM] (protein specific)

Localization (protein specific)

Metals/Ions (protein specific)

Molecular Weight [Da] (protein specific)

Natural Substrates/ Products (Substrates) (protein specific)

Organic Solvent Stability (protein specific)

Oxidation Stability (protein specific)

Posttranslational Modification (protein specific)

Purification (Commentary) (protein specific)

Renatured (Commentary) (protein specific)

Source Tissue (protein specific)

Specific Activity [micromol/min/mg] (protein specific)

Storage Stability (protein specific)

Substrates and Products (Substrate) (protein specific)

Subunits (protein specific)

Temperature Optimum [°C] (protein specific)

Temperature Range [°C] (protein specific)

Temperature Stability [°C] (protein specific)

Turnover Number [1/s] (protein specific)

pH Optimum (protein specific)

pH Range (protein specific)

pH Stability (protein specific)

pI Value (protein specific)

Expression

General Information

General Information (protein specific)

Expression (protein specific)

KCat/KM [mM/s]

KCat/KM [mM/s] (protein specific)

745216

Kazakov

The RimL transacetylase provi ...

Escherichia coli

J. Bacteriol.

196

3377-3385

2014

-

-

1

-

-

-

-

-

-

-

-

1

-

1

-

-

1

-

-

-

-

-

1

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

1

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

1

1

-

-

-

746391

Vetting

Crystal structure of RimI fro ...

Salmonella enterica subsp. enterica serovar Typhimurium

Protein Sci.

17

1781-1790

2008

-

-

1

1

-

-

1

-

-

-

-

1

-

1

-

-

1

-

-

-

-

-

1

-

1

-

-

-

-

-

-

-

-

1

-

-

-

-

1

-

1

-

-

-

1

1

-

-

-

-

1

-

-

-

1

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

745256

Tercero

MAK3 encodes an N-acetyltrans ...

Saccharomyces cerevisiae

J. Biol. Chem.

267

20277-20281

1992

-

-

-

-

-

-

-

-

-

-

-

1

-

1

-

-

1

-

-

-

-

-

1

-

1

-

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-

-

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-

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-

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1

-

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1

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

1

1

-

-

-

745779

Yoshikawa

Cloning and nucleotide sequen ...

Escherichia coli

Mol. Gen. Genet.

209

481-488

1987

-

-

1

-

-

-

-

-

-

-

-

1

-

1

-

-

-

-

-

-

-

-

1

2

1

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

1

2

-

-

-

-

-

-

-

-

-

-

-

-

-

-

745777

Isono

-

Ribosomal protein modificatio ...

Escherichia coli

Mol. Gen. Genet.

177

645-651

1980

-

-

-

-

-

-

-

-

-

-

-

1

-

1

-

-

-

-

-

-

-

-

1

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

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-

-

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-

-

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-