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Literature summary for 2.3.1.268 extracted from

  • Loebs, A.K.; Schwartz, C.; Thorwall, S.; Wheeldon, I.
    Highly multiplexed CRISPRi repression of respiratory functions enhances mitochondrial localized ethyl acetate biosynthesis in Kluyveromyces marxianus (2018), ACS Synth. Biol., 7, 2647-2655 .
    View publication on PubMed
Search for more literature for 2.3.1.268

Cloned(Commentary)

Cloned (Comment) Organism
gene EAT1, recombinant expression of N-terminally truncated mutants Eat1(DELTA1-14) and Eat1(DELTA1-19) in Saccharomyces cerevisiae and Kluyveromyces marxianus, recombinant expression of GFP-tagged wild-type enzyme in Kluyveromyces marxianus Kluyveromyces marxianus

Protein Variants

Protein Variants Comment Organism
additional information construction of an Eat1 knockout mutant and of N-terminally truncated mutants. Modulation of expression of the TCA cycle and electron transport chain genes using a highly multiplexed CRISPRi approach, simultaneous knockdown of ACO2b, SDH2, RIP1, and MSS51 results in a 3.8fold increase in ethyl acetate productivity over the already high natural capacity. Mitochondrial localization is partially lost when the first 14 amino acids are removed with complete cytosolic expression resulting from truncation at the predicted cut site, i.e. mutants Eat1(DELTA1-14) and Eat1(DELTA1-19) Kluyveromyces marxianus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion the N-terminal domain of enzyme Eat1 is responsible for targeting Kluyveromyces marxianus 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ethanol + acetyl-CoA Kluyveromyces marxianus
-
 ethyl acetate + CoA
-
 ?
ethanol + acetyl-CoA Kluyveromyces marxianus NBRC 104275
-
 ethyl acetate + CoA
-
 ?
ethanol + acetyl-CoA Kluyveromyces marxianus BCC 29191
-
 ethyl acetate + CoA
-
 ?
ethanol + acetyl-CoA Kluyveromyces marxianus DMKU3-1042
-
 ethyl acetate + CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Kluyveromyces marxianus W0T4A7
-
-
Kluyveromyces marxianus BCC 29191 W0T4A7
-
-
Kluyveromyces marxianus DMKU3-1042 W0T4A7
-
-
Kluyveromyces marxianus NBRC 104275 W0T4A7
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ethanol + acetyl-CoA
-
 Kluyveromyces marxianus ethyl acetate + CoA
-
 ?
ethanol + acetyl-CoA
-
 Kluyveromyces marxianus NBRC 104275 ethyl acetate + CoA
-
 ?
ethanol + acetyl-CoA
-
 Kluyveromyces marxianus BCC 29191 ethyl acetate + CoA
-
 ?
ethanol + acetyl-CoA
-
 Kluyveromyces marxianus DMKU3-1042 ethyl acetate + CoA
-
 ?

Synonyms

Synonyms Comment Organism
alcohol acetyltransferase
-
 Kluyveromyces marxianus
eat1
-
 Kluyveromyces marxianus

Cofactor

Cofactor Comment Organism Structure
acetyl-CoA
-
 Kluyveromyces marxianus

General Information

General Information Comment Organism
malfunction modulation of expression of the TCA cycle and electron transport chain genes using a highly multiplexed CRISPRi approach, simultaneous knockdown of ACO2b, SDH2, RIP1, and MSS51 results in a 3.8fold increase in ethyl acetate productivity over the already high natural capacity Kluyveromyces marxianus
metabolism the enzyme is involved in the the primary pathways of precursor and acetate ester biosynthesis Kluyveromyces marxianus
physiological function the alcohol acetyltransferase Eat1 is the critical enzyme for ethyl, isoamyl, and phenylethyl acetate production. Ethyl acetate is formed from the condensation of ethanol and acetyl-CoA. Mitochondrial localization of Eat1 is necessary for high ethyl acetate production Kluyveromyces marxianus