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Literature summary for 2.3.1.28 extracted from
- Purification and some properties of a chloramphenicol acetyltransferase mediated by plasmids from Vibrio anguillarum (1988), J. Biochem., 104, 131-135.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| plasmid Rms418 is transferred from Vibrio anguillarum to Escherichia coli K-12 | Vibrio anguillarum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5,5'-dithiobis(2-nitrobenzoic acid) | 0.025 mM, 82% inhibition | Vibrio anguillarum |  |
| EDTA | 0.1 mM, 24% inhibition | Vibrio anguillarum | |
| Hg2+ | 0.01 mM, complete inhibition | Vibrio anguillarum | |
| I2 | 0.1 mM, 20% inhibition | Vibrio anguillarum | |
| PCMB | 0.1 mM, complete inhibition | Vibrio anguillarum | |
| Zn2+ | 0.1 mM, 15% inhibition | Vibrio anguillarum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0345 | - |
chloramphenicol | - |
Vibrio anguillarum | |
| 0.156 | - |
acetyl-CoA | - |
Vibrio anguillarum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 22000 | - |
3 * 22000, SDS-PAGE | Vibrio anguillarum |
| 69000 | - |
gel filtration | Vibrio anguillarum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vibrio anguillarum | - |
enzyme is encoded by plasmid Rms418 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| plasmid Rms418 is transferred from Vibrio anguillarum to E. coli K-12 | Vibrio anguillarum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 71.4 | - |
- |
Vibrio anguillarum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80 C, 20% glycerol, stable for more than 3 months | Vibrio anguillarum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + chloramphenicol | - |
Vibrio anguillarum | CoA + chloramphenicol 3-acetate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | 3 * 22000, SDS-PAGE | Vibrio anguillarum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 53 | - |
0.1 mM chloramphenicol and 0.1 mM acetyl-CoA, 50 mM Tris-HCl buffer, pH 7.8, half-life: 100 min | Vibrio anguillarum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
- |
Vibrio anguillarum |
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