Cloned (Comment) | Organism |
---|---|
cloning of mutant cat-86 in pTB361 and transformation of Escherichia coli JM109 | Bacillus pumilus |
Protein Variants | Comment | Organism |
---|---|---|
A203G | mutant enzyme is less stable than wild-type enzyme | Bacillus pumilus |
A203I | mutant enzyme is more thermostable than wild-type | Bacillus pumilus |
I191V | mutant enzyme is less stable than wild-type enzyme | Bacillus pumilus |
Y33F/A203V | mutant enzyme is more thermostable than wild-type | Bacillus pumilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.025 | - |
chloramphenicol | wild-type enzyme | Bacillus pumilus | |
0.028 | - |
acetyl-CoA | wild-type enzyme | Bacillus pumilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus pumilus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bacillus pumilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
330 | - |
- |
Bacillus pumilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + chloramphenicol | - |
Bacillus pumilus | CoA + chloramphenicol 3-acetate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cat-86 | - |
Bacillus pumilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
30 min, 82% loss of activity, wild-type enzyme | Bacillus pumilus |