Protein Variants | Comment | Organism |
---|---|---|
L158I | fluorinated mutant expressed in trifluoroleucine shows enhanced thermostability compared to CAT T (CAT expressed in trifluoroleucine), suggesting that trifluoroleucine at position 158 contributes to a portion of the observed loss in thermostability upon global fluorination. Relative activity: 89% (non-fluorinated mutant), 51.7% (fluorinated mutant) | Escherichia coli |
L208I | fluorinated mutant expressed in trifluoroleucine shows loss in thermostability | Escherichia coli |
L821I | fluorinated mutant expressed in trifluoroleucine shows loss in thermostability | Escherichia coli |
additional information | residue-specific incorporation of T into chloramphenicol acetyltransferase (CAT) results in a loss of thermostability. Relative activity: 34.6% (fluorinated CAT) | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
relative activity: 94.9% (wild-type), 34.6% (fluorinated CAT), 89% (mutant L158I), 51.7% (fluorinated mutant L158I) | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + chloramphenicol | - |
Escherichia coli | CoA + chloramphenicol 3-acetate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CAT | - |
Escherichia coli |
chloramphenicol acetyltransferase | - |
Escherichia coli |