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Literature summary for 2.3.1.280 extracted from
- Functional annotation and kinetic characterization of PhnO from Salmonella enterica (2006), Biochemistry, 45, 3033-3039 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli BL21 (DE3) star cells | Salmonella enterica subsp. enterica serovar Typhimurium |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
Salmonella enterica subsp. enterica serovar Typhimurium |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.05 | - |
malonyl-CoA | cosubstrate: (S)-1-aminoethylphosphonic acid, pH 7.5, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 0.06 | - |
acetyl-CoA | cosubstrate: (S)-1-aminoethylphosphonic acid, pH 7.5, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 0.07 | - |
propionyl-CoA | cosubstrate: (S)-1-aminoethylphosphonic acid, pH 7.5, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 0.17 | - |
(S)-1-aminoethylphosphonic acid | pH 7.5, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 1.7 | - |
(aminomethyl)phosphonate | pH 7.5, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 1.8 | - |
2-aminoethyl phosphonic acid | pH 7.5, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 4 | - |
3-aminopropyl phosphonic acid | pH 7.5, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 4.1 | - |
1-aminopropyl phosphonic acid | pH 7.5, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 12.5 | - |
1-aminobutyl phosphonic acid | pH 7.5, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 13 | - |
4-aminobutylphosphonic acid | pH 7.5, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 16.4 | - |
2-aminoethanesulfinic acid | pH 7.5, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 18.9 | - |
2-aminoethanesulfonic acid | pH 7.5, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | the enzyme requires a divalent metal ion for activity. It exhibits a preference for Ni2+ followed by Mn2+ and very poor activity with Mg2+ | Salmonella enterica subsp. enterica serovar Typhimurium | |
| Mn2+ | the enzyme requires a divalent metal ion for activity. It exhibits a preference for Ni2+ followed by Mn2+ and very poor activity with Mg2+ | Salmonella enterica subsp. enterica serovar Typhimurium | |
| Ni2+ | the enzyme requires a divalent metal ion for activity. It exhibits a preference for Ni2+ followed by Mn2+ and very poor activity with Mg2+ | Salmonella enterica subsp. enterica serovar Typhimurium | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 16499 | - |
electrospray ionization-mass spectrometry | Salmonella enterica subsp. enterica serovar Typhimurium |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + (aminomethyl)phosphonate | Salmonella enterica subsp. enterica serovar Typhimurium | - |
CoA + (acetamidomethyl)phosphonate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Salmonella enterica subsp. enterica serovar Typhimurium | Q8ZKE6 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + (aminomethyl)phosphonate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | CoA + (acetamidomethyl)phosphonate | - |
? | |
| acetyl-CoA + (aminomethyl)phosphonate | reaction proceeds via an ordered, sequential kinetic mechanism with acetyl-CoA binding first followed by aminoalkylphosphonate. Attack by the amine on the thioester of AcCoA generates the tetrahedral intermediate that collapses to generate the products | Salmonella enterica subsp. enterica serovar Typhimurium | CoA + (acetamidomethyl)phosphonate | - |
? | |
| acetyl-CoA + (S)-1-aminoethylphosphonic acid | - |
Salmonella enterica subsp. enterica serovar Typhimurium | CoA + ? | - |
? | |
| acetyl-CoA + 1-aminobutyl phosphonic acid | - |
Salmonella enterica subsp. enterica serovar Typhimurium | CoA + ? | - |
? | |
| acetyl-CoA + 1-aminopropyl phosphonic acid | - |
Salmonella enterica subsp. enterica serovar Typhimurium | CoA + ? | - |
? | |
| acetyl-CoA + 2-aminoethanesulfinic acid | - |
Salmonella enterica subsp. enterica serovar Typhimurium | CoA + ? | - |
? | |
| acetyl-CoA + 2-aminoethanesulfonic acid | - |
Salmonella enterica subsp. enterica serovar Typhimurium | CoA + ? | - |
? | |
| acetyl-CoA + 2-aminoethyl phosphonic acid | - |
Salmonella enterica subsp. enterica serovar Typhimurium | CoA + ? | - |
? | |
| acetyl-CoA + 3-aminopropyl phosphonic acid | - |
Salmonella enterica subsp. enterica serovar Typhimurium | CoA + ? | - |
? | |
| acetyl-CoA + 4-aminobutylphosphonic acid | - |
Salmonella enterica subsp. enterica serovar Typhimurium | CoA + ? | - |
? | |
| malonyl-CoA + (S)-1-aminoethylphosphonic acid | - |
Salmonella enterica subsp. enterica serovar Typhimurium | CoA + ? | - |
? | |
| propionyl-CoA + (S)-1-aminoethylphosphonic acid | - |
Salmonella enterica subsp. enterica serovar Typhimurium | CoA + ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| phnO | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.4 | - |
malonyl-CoA | cosubstrate: (S)-1-aminoethylphosphonic acid, pH 7.5, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 0.5 | - |
4-aminobutylphosphonic acid | pH 7.5, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 1 | - |
1-aminobutyl phosphonic acid | pH 7.5, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 5.4 | - |
3-aminopropyl phosphonic acid | pH 7.5, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 7 | - |
(aminomethyl)phosphonate | pH 7.5, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 7.4 | - |
1-aminopropyl phosphonic acid | pH 7.5, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 9 | - |
2-aminoethyl phosphonic acid | pH 7.5, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 13.1 | - |
acetyl-CoA | cosubstrate: (S)-1-aminoethylphosphonic acid, pH 7.5, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 13.3 | - |
(S)-1-aminoethylphosphonic acid | pH 7.5, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 14.7 | - |
propionyl-CoA | cosubstrate: (S)-1-aminoethylphosphonic acid, pH 7.5, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 14.8 | - |
2-aminoethanesulfonic acid | pH 7.5, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 20 | - |
2-aminoethanesulfinic acid | pH 7.5, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5 | - |
2-aminoethyl phosphonic acid | pH 7.5, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 120 | - |
2-aminoethanesulfinic acid | pH 7.5, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 140 | - |
3-aminopropyl phosphonic acid | pH 7.5, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 180 | - |
1-aminopropyl phosphonic acid | pH 7.5, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 210 | - |
propionyl-CoA | cosubstrate: (S)-1-aminoethylphosphonic acid, pH 7.5, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 220 | - |
acetyl-CoA | cosubstrate: (S)-1-aminoethylphosphonic acid, pH 7.5, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 380 | - |
4-aminobutylphosphonic acid | pH 7.5, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 410 | - |
(aminomethyl)phosphonate | pH 7.5, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 780 | - |
(S)-1-aminoethylphosphonic acid | pH 7.5, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 780 | - |
2-aminoethanesulfonic acid | pH 7.5, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 800 | - |
1-aminobutyl phosphonic acid | pH 7.5, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 800 | - |
malonyl-CoA | cosubstrate: (S)-1-aminoethylphosphonic acid, pH 7.5, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
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