Cloned (Comment) | Organism |
---|---|
expression of SAT1 gene as glutathione-S-transferase fusion protein in Escherichia coli DH5alpha, complementation of serine acetyltransferase mutant Escherichia coli strain JM39, single copy gene, amino acid sequence determination | Arabidopsis thaliana |
SAT1 is closely linked to SAT5, high DNA sequence homology | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + L-serine | Arabidopsis thaliana | - |
CoA + O-acetyl-L-serine | - |
? | |
acetyl-CoA + L-serine | Arabidopsis thaliana | regulatory role in cysteine biosynthesis | CoA + O-acetyl-L-serine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q39218 | gene SAT1-6, mitochondrial isozyme | - |
Arabidopsis thaliana | Q42588 | gene SAT1 | - |
Arabidopsis thaliana | Q42588 | gene SAT5 | - |
Escherichia coli | P0A9D4 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | SAT1 gene | Arabidopsis thaliana | - |
leaf | SAT1-6 gene, major part | Arabidopsis thaliana | - |
root | SAT1 gene | Arabidopsis thaliana | - |
root | SAT1-6 gene, minor part | Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + L-serine | - |
Escherichia coli | CoA + O-acetyl-L-serine | - |
? | |
acetyl-CoA + L-serine | - |
Arabidopsis thaliana | CoA + O-acetyl-L-serine | - |
? | |
acetyl-CoA + L-serine | regulatory role in cysteine biosynthesis | Arabidopsis thaliana | CoA + O-acetyl-L-serine | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 32000-35000, SDS-PAGE | Escherichia coli |
? | x * 32000-35000, SDS-PAGE | Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Arabidopsis thaliana |