Cloned (Comment) | Organism |
---|---|
expression of mutant enzymes in Escherichia coli | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
crystal structure of the enzyme with its inhibitor L-cysteine | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
A94T | activity is 160.2% of wild-type value, Ki for L-Cys is 5.7fold higher than wild-type value | Escherichia coli |
M256I | Ki for L-Cys is 24fold higher than wild-type value | Escherichia coli |
R89H/T90V/P93A/A94T | activity is 113.1% of wild-type value, Ki for L-Cys is 658fold higher than wild-type value | Escherichia coli |
R89P | activity is 95.2% of wild-type value, Ki for L-Cys is 700fold higher than wild-type value | Escherichia coli |
R89S/T90L | activity is 72.6% of wild-type value, Ki for L-Cys is 25fold higher than wild-type value | Escherichia coli |
R99T/T90R | activity is 65.5% of wild-type value, Ki for L-Cys is 7.5fold higher than wild-type value | Escherichia coli |
V95G/D96G | activity is 85.7% of wild-type value, Ki for L-Cys is 190fold higher than wild-type value | Escherichia coli |
V95L/D96P | activity is 87.5% of wild-type value, Ki for L-Cys is 850fold higher than wild-type value | Escherichia coli |
V95R/D96P | activity is 42.5% of wild-type value, Ki for L-Cys is 1583fold higher than wild-type value | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-Cys | - |
Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + L-serine | Escherichia coli | first step of L-cysteine synthesis | CoA + O-acetyl-L-serine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0A9D4 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.715 | - |
25°C, pH 7.5, mutant enzyme V95R/D96P | Escherichia coli |
1.067 | - |
25°C, pH 7.5, mutant enzyme M256I | Escherichia coli |
1.1 | - |
25°C, pH 7.5, mutant enzyme R99T/T90R | Escherichia coli |
1.22 | - |
25°C, pH 7.5, mutant enzyme R89S/T90L | Escherichia coli |
1.44 | - |
25°C, pH 7.5, mutant enzyme V95G/D96G | Escherichia coli |
1.47 | - |
25°C, pH 7.5, mutant enzyme V95L/D96P | Escherichia coli |
1.6 | - |
25°C, pH 7.5, mutant enzyme R89P | Escherichia coli |
1.68 | - |
25°C, pH 7.5, wild-type enzyme | Escherichia coli |
1.9 | - |
25°C, pH 7.5, mutant enzyme R89H/T90V/P93A/A94T | Escherichia coli |
2.69 | - |
25°C, pH 7.5, mutant enzyme A94T | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + L-serine | first step of L-cysteine synthesis | Escherichia coli | CoA + O-acetyl-L-serine | - |
? |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0006 | - |
L-Cys | 25°C, pH 7.5, wild-type enzyme | Escherichia coli | |
0.0034 | - |
L-Cys | 25°C, pH 7.5, mutant enzyme A94T | Escherichia coli | |
0.0045 | - |
L-Cys | 25°C, pH 7.5, mutant enzyme R99T/T90R | Escherichia coli | |
0.0145 | - |
L-Cys | 25°C, pH 7.5, mutant enzyme M256I | Escherichia coli | |
0.015 | - |
L-Cys | 25°C, pH 7.5, mutant enzyme R89S/T90L | Escherichia coli | |
0.114 | - |
L-Cys | 25°C, pH 7.5, mutant enzyme V95G/D96G | Escherichia coli | |
0.395 | - |
L-Cys | 25°C, pH 7.5, mutant enzyme R89H/T90V/P93A/A94T | Escherichia coli | |
0.42 | - |
L-Cys | 25°C, pH 7.5, mutant enzyme R89P | Escherichia coli | |
0.51 | - |
L-Cys | 25°C, pH 7.5, mutant enzyme V95L/D96P | Escherichia coli | |
0.95 | - |
L-Cys | 25°C, pH 7.5, mutant enzyme V95R/D96P | Escherichia coli |