Application | Comment | Organism |
---|---|---|
medicine | inhibition of enzyme blocks growth of parasite trophozoites but not of mouse cell culture | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-amino-9-(naphthalen-2-yl)-9H-purin-6-ol | 0.05 microg/microl showing 38% inhibition in the presence of 0.10 mM acetyl-CoA, binding to active site | Escherichia coli | |
2-amino-9-naphthalen-2-yl-9H-purin-6-ol | - |
Entamoeba histolytica | |
3-oxo-2-phenyl-3,5-dihydro-2H-pyrazolo[3,4-d]thieno[2,3-b]pyridine-7-carboxylate | most potent inhibitor, 0.05 microg/microl showing 50% inhibition in the presence of 0.10 mM acetyl-CoA, binding to active site, increasing Km, decreasing Vmax | Escherichia coli | |
3-oxo-2-phenyl-3,5-dihydro-2H-pyrazolo[3,4-d]thieno[2,3-b]pyridine-7-carboxylic acid | - |
Entamoeba histolytica | |
4,10-dihydroxy-1,7-phenanthroline-3,9-dicarboxylate | 0.05 microg/microl showing 24% inhibition in the presence of 0.10 mM acetyl-CoA, binding to active site | Escherichia coli | |
4,10-dihydroxy-1,7-phenanthroline-3,9-dicarboxylic acid | - |
Entamoeba histolytica | |
additional information | no growth inhibition of mouse cell culture with 4,10-dihydroxy-1,7-phenanthroline-3,9-dicarboxylic acid, 2-amino-9-naphthalen-2-yl-9H-purin-6-ol, or 3-oxo-2-phenyl-3,5-dihydro-2H-pyrazolo[3,4-d]thieno[2,3-b]pyridine-7-carboxylic acid in a MTT assay | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-serine + acetyl-CoA | Mus musculus | - |
O-acetyl-L-serine + CoA | - |
? | |
L-serine + acetyl-CoA | Escherichia coli | - |
O-acetyl-L-serine + CoA | - |
? | |
L-serine + acetyl-CoA | Entamoeba histolytica | - |
O-acetyl-L-serine + CoA | - |
? | |
L-serine + acetyl-CoA | Escherichia coli DH5-alpha | - |
O-acetyl-L-serine + CoA | - |
? | |
L-serine + acetyl-CoA | Entamoeba histolytica HMM1:IMSS | - |
O-acetyl-L-serine + CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Entamoeba histolytica | Q9U8X2 | trophozoite | - |
Entamoeba histolytica HMM1:IMSS | Q9U8X2 | trophozoite | - |
Escherichia coli | P0A9D4 | - |
- |
Escherichia coli DH5-alpha | P0A9D4 | - |
- |
Mus musculus | - |
mouse cell line | - |
Purification (Comment) | Organism |
---|---|
cells centrifuged, resuspended in 0.01 M Tris-HCl, pH 7.5, sonicated, centrifuged, supernatant subjected to streptomycin sulfate (10%) and ammonium sulfate (70%) precipitation, precipitated protein dissolved in same buffer, desalted on a G-25 column | Mus musculus |
cells centrifuged, resuspended in 0.01 M Tris-HCl, pH 7.5, sonicated, centrifuged, supernatant subjected to streptomycin sulfate (10%) and ammonium sulfate (70%) precipitation, precipitated protein dissolved in same buffer, desalted on a G-25 column | Escherichia coli |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
Vero cell | C-1008 cell line | Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-serine + acetyl-CoA | - |
Mus musculus | O-acetyl-L-serine + CoA | - |
? | |
L-serine + acetyl-CoA | - |
Escherichia coli | O-acetyl-L-serine + CoA | - |
? | |
L-serine + acetyl-CoA | - |
Entamoeba histolytica | O-acetyl-L-serine + CoA | - |
? | |
L-serine + acetyl-CoA | - |
Escherichia coli DH5-alpha | O-acetyl-L-serine + CoA | - |
? | |
L-serine + acetyl-CoA | - |
Entamoeba histolytica HMM1:IMSS | O-acetyl-L-serine + CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | 3 * x, predicted from homology modeling with Escherichia coli enzyme crystal structure | Entamoeba histolytica |
Synonyms | Comment | Organism |
---|---|---|
SATase | - |
Mus musculus |
SATase | - |
Escherichia coli |
SATase | - |
Entamoeba histolytica |
serine acetyltransferase | - |
Mus musculus |
serine acetyltransferase | - |
Escherichia coli |
serine acetyltransferase | - |
Entamoeba histolytica |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.042 | - |
3-oxo-2-phenyl-3,5-dihydro-2H-pyrazolo[3,4-d]thieno[2,3-b]pyridine-7-carboxylic acid | 72 micromol, 0.1 M Tris-HCl, pH 7.5, room temperature | Escherichia coli |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00061 | - |
count of viable trophozoites after 72 h compared to control measured with 32.16, 64.32, and 160.8 microM inhibitor | Entamoeba histolytica | 3-oxo-2-phenyl-3,5-dihydro-2H-pyrazolo[3,4-d]thieno[2,3-b]pyridine-7-carboxylic acid | |
0.00107 | - |
count of viable trophozoites after 72 h compared to control measured with 32.16, 64.32, and 160.8 microM inhibitor | Entamoeba histolytica | 4,10-dihydroxy-1,7-phenanthroline-3,9-dicarboxylic acid | |
0.00152 | - |
count of viable trophozoites after 72 h compared to control measured with 32.16, 64.32, and 160.8 microM inhibitor | Entamoeba histolytica | 2-amino-9-naphthalen-2-yl-9H-purin-6-ol | |
0.072 | - |
72 micromol, 0.1 M Tris-HCl, pH 7.5, room temperature | Escherichia coli | 3-oxo-2-phenyl-3,5-dihydro-2H-pyrazolo[3,4-d]thieno[2,3-b]pyridine-7-carboxylic acid |
General Information | Comment | Organism |
---|---|---|
malfunction | compounds that (partially) block the Escherichia coli enzyme activity block the growth of Entamoeba histolytica trophozoites but not mammalian cells | Mus musculus |
malfunction | compounds that (partially) block the Escherichia coli enzyme activity block the growth of Entamoeba histolytica trophozoites but not mammalian cells | Escherichia coli |
malfunction | compounds that (partially) block the Escherichia coli enzyme activity block the growth of Entamoeba histolytica trophozoites but not mammalian cells | Entamoeba histolytica |