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Literature summary for 2.3.1.310 extracted from

  • Weidenweber, S.; Schuehle, K.; Lippert, M.L.; Mock, J.; Seubert, A.; Demmer, U.; Ermler, U.; Heider, J.
    Finis tolueni a new type of thiolase with an integrated Zn-finger subunit catalyzes the final step of anaerobic toluene metabolism (2022), FEBS J., FEHLT, 0000 .
    View publication on PubMed
Search for more literature for 2.3.1.310

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pseudomonas stutzeri Geobacter metallireducens

Crystallization (Commentary)

Crystallization (Comment) Organism
structure of BbsAB with and without bound CoA at is determined at 1.7 A and 2.0 A resolution Geobacter metallireducens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.114
-
 benzoyl-CoA calculated, kinetic model: ternary complex mechanism, pH and temperature not specified in the publication Geobacter metallireducens
0.31
-
 succinyl-CoA calculated, kinetic model: ternary complex mechanism, pH and temperature not specified in the publication Geobacter metallireducens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
 BbsA, gel filtration Geobacter metallireducens
91000
-
 BbsB, gel filtration Geobacter metallireducens
125000
-
 holoenzyme, gel filtration Geobacter metallireducens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-2-benzoylsuccinyl-CoA + CoA Geobacter metallireducens the enzyme catalyzes the final step of anaerobic toluene metabolism benzoyl-CoA + succinyl-CoA
-
 r
(S)-2-benzoylsuccinyl-CoA + CoA Geobacter metallireducens ATCC 53774 the enzyme catalyzes the final step of anaerobic toluene metabolism benzoyl-CoA + succinyl-CoA
-
 r

Organism

Organism UniProt Comment Textmining
Geobacter metallireducens Q39VG2 AND Q39VG1 Q39VG2: bbsA, Q39VG1: bbsB
-
Geobacter metallireducens ATCC 53774 Q39VG2 AND Q39VG1 Q39VG2: bbsA, Q39VG1: bbsB
-

Purification (Commentary)

Purification (Comment) Organism
the BbsAB complex is purified in one step via strep-tag affinity chromatography without apparent splitting up into the respective subunits, yielding 50-60 mg pure protein per 10 g wet cell mass, further purified by size exclusion chromatography Geobacter metallireducens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-2-benzoylsuccinyl-CoA + CoA the enzyme catalyzes the final step of anaerobic toluene metabolism Geobacter metallireducens benzoyl-CoA + succinyl-CoA
-
 r
(S)-2-benzoylsuccinyl-CoA + CoA thioester-dependent Claisen-type mechanism Geobacter metallireducens benzoyl-CoA + succinyl-CoA
-
 r
(S)-2-benzoylsuccinyl-CoA + CoA the enzyme catalyzes the final step of anaerobic toluene metabolism Geobacter metallireducens ATCC 53774 benzoyl-CoA + succinyl-CoA
-
 r
(S)-2-benzoylsuccinyl-CoA + CoA thioester-dependent Claisen-type mechanism Geobacter metallireducens ATCC 53774 benzoyl-CoA + succinyl-CoA
-
 r
benzoyl-CoA + succinyl-CoA
-
 Geobacter metallireducens (S)-2-benzoylsuccinyl-CoA + CoA
-
 r
benzoyl-CoA + succinyl-CoA
-
 Geobacter metallireducens ATCC 53774 (S)-2-benzoylsuccinyl-CoA + CoA
-
 r

Synonyms

Synonyms Comment Organism
(S)-2-benzoylsuccinyl-CoA thiolase
-
 Geobacter metallireducens
bbsAB
-
 Geobacter metallireducens

General Information

General Information Comment Organism
metabolism the enzyme catalyzes the final step of anaerobic toluene metabolism Geobacter metallireducens