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Literature summary for 2.3.1.36 extracted from

  • Yow, G.Y.; Uo, T.; Yoshimura, T.; Esaki, N.
    D-amino acid N-acetyltransferase of Saccharomyces cerevisiae: a close homologue of histone acetyltransferase Hpa2p acting exclusively on free D-amino acids (2004), Arch. Microbiol., 182, 396-403.
    View publication on PubMed
Search for more literature for 2.3.1.36

Cloned(Commentary)

Cloned (Comment) Organism
gene HPA3, functional overexpression in Escherichia coli Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
CoA competitive against acetyl-CoA, noncompetitive against D-tryptophan Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics Saccharomyces cerevisiae
0.034
-
 D-serine 30°C, recombinant enzyme Saccharomyces cerevisiae
0.054
-
 D-alanine 30°C, recombinant enzyme Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
18000
-
 x * 21000, recombinant intact Hpa3p, SDS-PAGE, x * 18000, recombinant N-terminally truncated enzyme, SDS-PAGE Saccharomyces cerevisiae
21000
-
 x * 21000, recombinant intact Hpa3p, SDS-PAGE, x * 18000, recombinant N-terminally truncated enzyme, SDS-PAGE Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + a D-amino acid Saccharomyces cerevisiae
-
 CoA + an N-acetyl-D-amino acid
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
 gene HPA3
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme from dry yeast, to homogeneity in several steps, recombinant enzyme from Escherichia coli in a procedure similar to the purification of the native enzyme to 95% purity Saccharomyces cerevisiae

Reaction

Reaction Comment Organism Reaction ID
acetyl-CoA + a D-amino acid = CoA + an N-acetyl-D-amino acid ordered bi bi mechanism with acetyl-CoA being the first substrate to be bound and CoA the last product to be released, involving acetylation of Cys304 Saccharomyces cerevisiae
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 substrate specificity Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + a D-amino acid
-
 Saccharomyces cerevisiae CoA + an N-acetyl-D-amino acid
-
 ?
acetyl-CoA + a D-amino acid substrate specificity, overview Saccharomyces cerevisiae CoA + an N-acetyl-D-amino acid
-
 ?
acetyl-CoA + D-alanine
-
 Saccharomyces cerevisiae CoA + N-acetyl-D-alanine
-
 ?
acetyl-CoA + D-alpha-aminobutyrate
-
 Saccharomyces cerevisiae CoA + N-acetyl-D-alpha-aminobutyrate
-
 ?
acetyl-CoA + D-arginine
-
 Saccharomyces cerevisiae CoA + N-acetyl-D-arginine
-
 ?
acetyl-CoA + D-asparagine
-
 Saccharomyces cerevisiae CoA + N-acetyl-D-asparagine
-
 ?
acetyl-CoA + D-glutamine
-
 Saccharomyces cerevisiae CoA + N-acetyl-D-glutamine
-
 ?
acetyl-CoA + D-lysine
-
 Saccharomyces cerevisiae CoA + N-acetyl-D-lysine
-
 ?
acetyl-CoA + D-methionine
-
 Saccharomyces cerevisiae CoA + N-acetyl-D-methionine
-
 ?
acetyl-CoA + D-norleucine
-
 Saccharomyces cerevisiae CoA + N-acetyl-D-norleucine
-
 ?
acetyl-CoA + D-norvaline
-
 Saccharomyces cerevisiae CoA + N-acetyl-D-norvaline
-
 ?
acetyl-CoA + D-phenylalanine
-
 Saccharomyces cerevisiae CoA + N-acetyl-D-phenylalanine
-
 ?
acetyl-CoA + D-serine
-
 Saccharomyces cerevisiae CoA + N-acetyl-D-serine
-
 ?
acetyl-CoA + D-threonine
-
 Saccharomyces cerevisiae CoA + N-acetyl-D-threonine
-
 ?
acetyl-CoA + D-tryptophan
-
 Saccharomyces cerevisiae CoA + N-acetyl-D-tryptophan
-
 ?
acetyl-CoA + D-valine
-
 Saccharomyces cerevisiae CoA + N-acetyl-D-valine
-
 ?
additional information no activity with L-lysine, D-proline, and D-aspartate, D-glutamate is a poor substrate Saccharomyces cerevisiae ?
-
 ?

Subunits

Subunits Comment Organism
? x * 21000, recombinant intact Hpa3p, SDS-PAGE, x * 18000, recombinant N-terminally truncated enzyme, SDS-PAGE Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
DNT
-
 Saccharomyces cerevisiae
Hpa3p
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Saccharomyces cerevisiae