Cloned (Comment) | Organism |
---|---|
expression of His-tagged wild-type, mutant K313A, mutant R149A and dimer mutant K313A/R149A, each subunit from 1 plasmid, in Escherichia coli hemA- mutant | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
C145R | mutant strain G205, lacks enzyme activity, but can complement mutant strain Ole3 with mutation G344C | Saccharomyces cerevisiae |
K313A | mutation site located at the active site of 1 subunit, functional complementation of Escherichia coli mutant strain hemA-, no activity | Mus musculus |
K313A/R149A | each mutation site located on 1 subunit, 2 plasmids, coexpression of the dimer in Escherichia coli hemA-, functional complementation, 26% activity compared to wild-type | Mus musculus |
N157Y/N162S | mutant strain G101, lacks enzyme activity, but can complement mutant strain G220 with mutation T452R | Saccharomyces cerevisiae |
R149A | mutation site located at the active site of 1 subunit, functional complementation of Escherichia coli mutant strain hemA-, no activity | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.52 | - |
succinyl-CoA | recombinant mutant dimer K149A/K313A | Mus musculus | |
1.82 | - |
succinyl-CoA | - |
Mus musculus | |
6.95 | - |
glycine | recombinant mutant dimer K149A/K313A | Mus musculus | |
8.39 | - |
glycine | - |
Mus musculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
amino acid sequence alignment | Mus musculus |
additional information | - |
amino acid sequence alignment | Saccharomyces cerevisiae |
56000 | - |
2 * 56000, SDS-PAGE | Mus musculus |
112000 | - |
gel filtration | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
succinyl-CoA + glycine | Mus musculus | rate-limiting enzyme of heme biosynthesis | 5-aminolevulinate + CoA + CO2 | - |
? | |
succinyl-CoA + glycine | Saccharomyces cerevisiae | rate-limiting enzyme of heme biosynthesis | 5-aminolevulinate + CoA + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
gene hemA | - |
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant of His-tagged wild-type and mutants from E. coli | Mus musculus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2 | Tyr121, Asp279, Arg439, and Lys313 are involved in substrate and cofactor binding, mechanism, subunit localisation | Mus musculus | |
succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2 | the active site is located at the subunit interface and contains catalytically essential residues from the two subunits | Mus musculus | |
succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2 | the active site is located at the subunit interface and contains catalytically essential residues from the two subunits | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
succinyl-CoA + glycine | - |
Mus musculus | 5-aminolevulinate + CoA + CO2 | - |
? | |
succinyl-CoA + glycine | - |
Saccharomyces cerevisiae | 5-aminolevulinate + CoA + CO2 | - |
? | |
succinyl-CoA + glycine | rate-limiting enzyme of heme biosynthesis | Mus musculus | 5-aminolevulinate + CoA + CO2 | - |
? | |
succinyl-CoA + glycine | rate-limiting enzyme of heme biosynthesis | Saccharomyces cerevisiae | 5-aminolevulinate + CoA + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Saccharomyces cerevisiae |
dimer | 2 * 56000, SDS-PAGE | Mus musculus |
More | the active site is located at the subunit interface and contains catalytically essential residues from the two subunits | Mus musculus |
More | the active site is located at the subunit interface and contains catalytically essential residues from the two subunits | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Mus musculus | |
pyridoxal 5'-phosphate | - |
Saccharomyces cerevisiae |