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Literature summary for 2.3.1.38 extracted from
- A Streptomyces collinus thiolase with novel acetyl-CoA:acyl carrier protein transacylase activity (2001), Biochemistry, 40, 11955-11964.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloning and sequencing of fadA gene, expression in Escherichia coli of wild-type and C92S mutant | Streptomyces collinus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C92S | no remaining activity acetyl-CoA:acyl carrier protein transacylase activity, but remaining strongly reduced thiolase activity | Streptomyces collinus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| iodoacetamide | acetyl-CoA protects; strong | Streptomyces collinus |  |
| thiolactomycin | - |
Streptomyces collinus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0043 | - |
acetyl-CoA | native wild-type enzyme | Streptomyces collinus | |
| 0.0087 | - |
acetyl-CoA | recombinant wild-type enzyme | Streptomyces collinus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
amino acid sequence, comparison | Streptomyces collinus |
| 45000 | - |
4 * 45000, SDS-PAGE | Streptomyces collinus |
| 185000 | - |
gel filtration | Streptomyces collinus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces collinus | - |
gene fadA | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native wild-type and recombinant wild-type and mutant from E. coli | Streptomyces collinus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.022 | - |
purified native wild-type enzyme | Streptomyces collinus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + [acyl-carrier protein] | specific for acetyl-CoA, utilizes [acyl-carrier-protein] of diverse origins with differing affinities | Streptomyces collinus | CoA + acetyl-[acyl-carrier protein] | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 45000, SDS-PAGE | Streptomyces collinus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| acetyl-CoA:acyl carrier protein transacylase | - |
Streptomyces collinus |
| ACT | - |
Streptomyces collinus |
| More | cf. EC 2.3.1.85 and EC 2.3.1.86 | Streptomyces collinus |
| More | enzyme has both a acetyl-CoA:acyl carrier protein transacylase activity and a much higher thiolase activity | Streptomyces collinus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Streptomyces collinus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Streptomyces collinus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0225 | - |
acetyl-CoA | recombinant wild-type, crude Streptomyces glaucescens [acyl-carrier-protein] | Streptomyces collinus | |
| 0.0508 | - |
acetyl-CoA | native wild-type, crude E. coli [acyl-carrier-protein] | Streptomyces collinus | |
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Release 2023.1 (January 2023)
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