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Literature summary for 2.3.1.4 extracted from

  • Brockhausen, I.; Nair, D.G.; Chen, M.; Yang, X.; Allingham, J.S.; Szarek, W.A.; Anastassiades, T.
    Human acetyl-CoA glucosamine-6-phosphate N-acetyltransferase 1 has a relaxed donor specificity and transfers acyl groups up to four carbons in length (2016), Biochem. Cell Biol., 94, 197-204 .
    View publication on PubMed
Search for more literature for 2.3.1.4

Application

Application Comment Organism
analysis development of a fluorescence assay where reactive thiols of CoA, generated from the acyl transfer reaction, are monitored using monobromobimane Homo sapiens

Protein Variants

Protein Variants Comment Organism
C157A mutant shows activities of both acetyl- and butyryltransfer, in the range of the wild type enzyme activity Homo sapiens
L138A mutant shows activities of both acetyl- and butyryltransfer, in the range of the wild type enzyme activity. Ratio of butanoyl-to-acetyltransferase activity is increased to 1 Homo sapiens
L155A mutant shows activities of both acetyl- and butyryltransfer, in the range of the wild type enzyme activity Homo sapiens
L155A/N161A mutant shows activities of both acetyl- and butyryltransfer, in the range of the wild type enzyme activity Homo sapiens
L155G mutant shows activities of both acetyl- and butyryltransfer, in the range of the wild type enzyme activity. Ratio of butanoyl-to-acetyltransferase activity is increased to 0.9 Homo sapiens
L155G/N161A mutant shows activities of both acetyl- and butyryltransfer, in the range of the wild type enzyme activity. Ratio of butanoyl-to-acetyltransferase activity is increased to 0.85 Homo sapiens
L158A mutant shows activities of both acetyl- and butyryltransfer, in the range of the wild type enzyme activity Homo sapiens
L158A/N161A mutant shows activities of both acetyl- and butyryltransfer, in the range of the wild type enzyme activity Homo sapiens
L158G mutant shows activities of both acetyl- and butyryltransfer, in the range of the wild type enzyme activity Homo sapiens
L158G/N161A mutant shows activities of both acetyl- and butyryltransfer, in the range of the wild type enzyme activity Homo sapiens
N161A mutant shows activities of both acetyl- and butyryltransfer, in the range of the wild type enzyme activity Homo sapiens
P159A mutant shows activities of both acetyl- and butyryltransfer, in the range of the wild type enzyme activity Homo sapiens
Y165A mutant shows activities of both acetyl- and butyryltransfer, in the range of the wild type enzyme activity. Ratio of butanoyl-to-acetyltransferase activity is increased to 0.9 Homo sapiens
Y165T mutant shows activities of both acetyl- and butyryltransfer, in the range of the wild type enzyme activity. Ratio of butanoyl-to-acetyltransferase activity is increased to 0.9 Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibitory: 1,22-bis(3-methyl-1H-imidazolium-1-yl)docosane dimesylate, N-butanoylglucosaminex02beta-S-2-naphthyl Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.13
-
 propionyl-CoA pH 7.6, 37°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q96EK6
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + D-glucosamine 6-phosphate
-
 Homo sapiens CoA + N-acetyl-D-glucosamine 6-phosphate
-
 ?
butanoyl-CoA + D-glucosamine 6-phosphate
-
 Homo sapiens CoA + N-butanoyl-D-glucosamine 6-phosphate
-
 ?
isobutanoyl-CoA + D-glucosamine 6-phosphate
-
 Homo sapiens CoA + N-isobutanoyl-D-glucosamine 6-phosphate
-
 ?
additional information ratio of butanoyl-to-acetyltransferase activity for wild-type: 0.6. No substrates: isovaleryl-CoA and decanoyl-CoA Homo sapiens ?
-
-
propionyl-CoA + D-glucosamine 6-phosphate
-
 Homo sapiens CoA + N-propionyl-D-glucosamine 6-phosphate
-
 ?