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Literature summary for 2.3.1.42 extracted from

  • Jones, C.L.; Hajra, A.K.
    Solubilization and partial purification of dihydroxyacetone-phosphate acyltransferase from guinea pig liver (1983), Arch. Biochem. Biophys., 226, 155-165.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
asolectin activation, i.e. crude soybean phospholipid preparation containing phosphatidylcholine, phosphatidylethanolamine and phosphatidylinositol Cavia porcellus
Phospholipid crude peroxisomal phospholipids Cavia porcellus
Phospholipid at low concentration: phosphatidylserine and phosphatidylethanolamine, both inhibitory at high concentration Cavia porcellus
Phospholipid phosphatidylglycerol Cavia porcellus
Phospholipid activation, e.g. phosphatidylcholine, dilauroylphosphatidylcholine Cavia porcellus

General Stability

General Stability Organism
asolectin stabilizes against inactivation during freezing and thawing Cavia porcellus
DEAE-Sephacel chromatography inactivates Cavia porcellus
freezing inactivates Cavia porcellus
isoelectric focusing inactivates Cavia porcellus

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithio-bis(2-nitrobenzoate) i.e. DTNB Cavia porcellus
Cd2+
-
Cavia porcellus
cholate above 2 mg/ml Cavia porcellus
Cu2+
-
Cavia porcellus
iodoacetamide 5 mM, weak Cavia porcellus
p-chloromercuriphenylsulfonic acid 5 mM Cavia porcellus
phosphatidic acid
-
Cavia porcellus
phosphatidylethanolamine above 1.5 mM Cavia porcellus
phosphatidylinositol
-
Cavia porcellus
phosphatidylserine above 1.5 mM Cavia porcellus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Cavia porcellus
0.065
-
palmitoyl-CoA
-
Cavia porcellus
0.1 0.5 dihydroxyacetone phosphate in presence of asolectin Cavia porcellus

Localization

Localization Comment Organism GeneOntology No. Textmining
peroxisome
-
Cavia porcellus 5777
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no activation by Ca2+ Cavia porcellus
additional information no activation by Mg2+, partially purified Cavia porcellus
additional information no activation by Mn2+, partially purified Cavia porcellus

Organism

Organism UniProt Comment Textmining
Cavia porcellus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial, solubilized with sodium cholate-KCl Cavia porcellus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Cavia porcellus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.106
-
-
Cavia porcellus
0.212
-
-
Cavia porcellus

Storage Stability

Storage Stability Organism
2-4°C, 30-50% loss of activity after 1 month, DTT stabilizes during storage Cavia porcellus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acyl-CoA + dihydroxyacetone phosphate i.e. glycerone phosphate, saturated acyl-CoAs preferred over unsaturated Cavia porcellus CoA + acyldihydroxyacetone phosphate i.e. acylglycerone phosphate r
arachidonoyl-CoA + dihydroxyacetone phosphate i.e. eicosatetraenoyl-CoA, poor substrate Cavia porcellus CoA + arachidonoyldihydroxyacetone phosphate
-
r
linoleoyl-CoA + dihydroxyacetone phosphate
-
Cavia porcellus CoA + linoleoyldihydroxyacetone phosphate
-
r
oleoyl-CoA + dihydroxyacetone phosphate poor substrate Cavia porcellus CoA + oleoyldihydroxyacetone phosphate
-
r
palmitoyl-CoA + dihydroxyacetone phosphate best substrate Cavia porcellus CoA + monopalmitoyldihydroxyacetone phosphate
-
r
stearoyl-CoA + dihydroxyacetone phosphate acylation at 35% the rate of palmitoyl-CoA Cavia porcellus CoA + stearoyldihydroxyacetone phosphate
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Cavia porcellus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
48
-
t1/2: 15 min Cavia porcellus
51
-
t1/2: 15 min, with asolectin Cavia porcellus
60
-
10 min stable Cavia porcellus
60
-
intact peroxisomes Cavia porcellus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4
-
in presence of asolectin Cavia porcellus
7 8.2 broad Cavia porcellus

pH Range

pH Minimum pH Maximum Comment Organism
6 9 about half-maximal activity at pH 6.0 and pH 9.0, in the presence of asolectin Cavia porcellus