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Literature summary for 2.3.1.45 extracted from

  • Lewis, A.L.; Hensler, M.E.; Varki, A.; Nizet, V.
    The group B streptococcal sialic acid O-acetyltransferase is encoded by neuD, a conserved component of bacterial sialic acid biosynthetic gene clusters (2006), J. Biol. Chem., 281, 11186-11192.
    View publication on PubMed
Search for more literature for 2.3.1.45

Cloned(Commentary)

Cloned (Comment) Organism
gene neuD, DNA and amino acid sequence determination and analysis, phylogenetic analysis, homology modeling of NeuD Streptococcus sp.
gene neuD, DNA and amino acid sequence determination and analysis, phylogenetic analysis, homology modeling of NeuD, functional complementation of an enzyme-deficient neuD-mutant Streptococcus sp. strain Escherichia coli

Protein Variants

Protein Variants Comment Organism
F88C single nucleotide polymorphism, associated with variant group B streptococcal O-acetylation phenotype, overview Streptococcus sp.
K123A site-directed mutagenesis, complementation of the COH1DELTANeuD mutant with the K123A mutant of NeuD restores sialylation but not O-acetylation Streptococcus sp.
additional information construction of an enzyme-deficient mutant strain DELTANeuD by allelic replacement mutagenesis, the mutant is functionally complemented by the enzyme from Escherichia coli K1, encoded by gene neuD, the mutant is also analyzed for complementation by the streptococcal K123A mutant, the NeuD-K123A plasmid restores sialic acid biosynthesis to the DELTANeuD mutant, but not the O-acetylation of Neu5Ac Streptococcus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + N-acetylneuraminic acid Streptococcus sp.
-
 CoA + N-acetyl-9-O-acetyl-neuraminate
-
 ?
acetyl-CoA + N-acetylneuraminic acid Escherichia coli
-
 CoA + N-acetyl-9-O-acetyl-neuraminate
-
 ?
additional information Escherichia coli Escherichia coli K1, a leading cause of neonatal sepsis and meningitis, expresses a surface CPS containing Neu5Ac, further modified this Sia by O-acetylation ?
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli Q46674 strain ATCC 23511, gene neuD
-
Streptococcus sp.
-
 group B, serotype III GBS strain COH1, gene neuD
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + N-acetylneuraminic acid
-
 Streptococcus sp. CoA + N-acetyl-9-O-acetyl-neuraminate
-
 ?
acetyl-CoA + N-acetylneuraminic acid
-
 Escherichia coli CoA + N-acetyl-9-O-acetyl-neuraminate
-
 ?
additional information Escherichia coli K1, a leading cause of neonatal sepsis and meningitis, expresses a surface CPS containing Neu5Ac, further modified this Sia by O-acetylation Escherichia coli ?
-
 ?

Synonyms

Synonyms Comment Organism
group B streptococcal sialic acid O-acetyltransferase
-
 Streptococcus sp.
group B streptococcal sialic acid O-acetyltransferase
-
 Escherichia coli
NeuD
-
 Streptococcus sp.
NeuD
-
 Escherichia coli
Sia O-acetyltransferase
-
 Streptococcus sp.
Sia O-acetyltransferase
-
 Escherichia coli

Cofactor

Cofactor Comment Organism Structure
acetyl-CoA
-
 Streptococcus sp.
acetyl-CoA
-
 Escherichia coli