Cloned (Comment) | Organism |
---|---|
improved overexpression in Escherichia coli bioF- strain R874 | Lysinibacillus sphaericus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0015 | - |
pimeloyl-CoA | - |
Lysinibacillus sphaericus | |
0.002 | - |
L-alanine | - |
Lysinibacillus sphaericus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-carboxyhexanoyl-CoA + L-alanine | Lysinibacillus sphaericus | i.e. pimeloyl-CoA | 8-amino-7-oxononanoate + CoA + CO2 | i.e. 7-oxo-8-amino-pelargonic acid | ir | |
6-carboxyhexanoyl-CoA + L-alanine | Lysinibacillus sphaericus | second step in biotin biosynthesis | 8-amino-7-oxononanoate + CoA + CO2 | i.e. 7-oxo-8-amino-pelargonic acid | ir | |
6-carboxyhexanoyl-CoA + L-alanine | Lysinibacillus sphaericus | reaction in biotin biosynthesis | 8-amino-7-oxononanoate + CoA + CO2 | i.e. 7-oxo-8-amino-pelargonic acid | ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lysinibacillus sphaericus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant from E. coli overproducing strain | Lysinibacillus sphaericus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
pimeloyl-[acyl-carrier protein] + L-alanine = 8-amino-7-oxononanoate + CO2 + holo-[acyl-carrier protein] | reaction mechanism | Lysinibacillus sphaericus | |
pimeloyl-[acyl-carrier protein] + L-alanine = 8-amino-7-oxononanoate + CO2 + holo-[acyl-carrier protein] | reaction mechanism, formation of the pyridoxal-phosphate-stabilized carbanion by abstraction of the C2-H proton of the alanine-pyridoxal-phosphate aldimine, followed by acylation and decarboxylation | Lysinibacillus sphaericus | |
pimeloyl-[acyl-carrier protein] + L-alanine = 8-amino-7-oxononanoate + CO2 + holo-[acyl-carrier protein] | reaction mechanism could be shared by all alpha-oxoamine synthases | Lysinibacillus sphaericus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-carboxyhexanoyl-CoA + L-alanine | i.e. pimeloyl-CoA | Lysinibacillus sphaericus | 8-amino-7-oxononanoate + CoA + CO2 | i.e. 7-oxo-8-amino-pelargonic acid | ir | |
6-carboxyhexanoyl-CoA + L-alanine | second step in biotin biosynthesis | Lysinibacillus sphaericus | 8-amino-7-oxononanoate + CoA + CO2 | i.e. 7-oxo-8-amino-pelargonic acid | ir | |
6-carboxyhexanoyl-CoA + L-alanine | reaction in biotin biosynthesis | Lysinibacillus sphaericus | 8-amino-7-oxononanoate + CoA + CO2 | i.e. 7-oxo-8-amino-pelargonic acid | ir |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
pimeloyl-CoA | - |
Lysinibacillus sphaericus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Lysinibacillus sphaericus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | dependent on | Lysinibacillus sphaericus | |
pyridoxal 5'-phosphate | incubation of enzyme with L-alanine but without pyridoxal-5'-phosphate lead to abortive transamination | Lysinibacillus sphaericus |