Cloned (Comment) | Organism |
---|---|
gene Rv1569, overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-KAPA | product inhibition | Mycobacterium tuberculosis | |
additional information | no inhibition by D-alanine | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | stopped-flow kinetics of enzyme interaction with L-alanine and D-alanine, steady-state kinetics and thermodynamics | Mycobacterium tuberculosis | |
0.0015 | - |
pimeloyl-CoA | pH 7.7, 25°C, recombinant enzyme | Mycobacterium tuberculosis | |
0.2015 | - |
D-alanine | pH 7.7, 25°C, recombinant enzyme, with pimeloyl-CoA | Mycobacterium tuberculosis | |
0.4675 | - |
L-alanine | pH 7.7, 25°C, recombinant enzyme, with pimeloyl-CoA | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
x * 42000, recombinant His-tagged enzyme, SDS-PAGE, x * 42249, recombinant His-tagged enzyme, mass spectrometry, x * 42194, amino acid sequence calculation | Mycobacterium tuberculosis |
42194 | - |
x * 42000, recombinant His-tagged enzyme, SDS-PAGE, x * 42249, recombinant His-tagged enzyme, mass spectrometry, x * 42194, amino acid sequence calculation | Mycobacterium tuberculosis |
42249 | - |
x * 42000, recombinant His-tagged enzyme, SDS-PAGE, x * 42249, recombinant His-tagged enzyme, mass spectrometry, x * 42194, amino acid sequence calculation | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-carboxyhexanoyl-CoA + L-alanine | Mycobacterium tuberculosis | first committed step in the biotin biosynthesis pathway | 8-amino-7-oxononanoate + CoA + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
gene Rv1569 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affiinity chromatography and gel filtration to homogeneity | Mycobacterium tuberculosis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
pimeloyl-[acyl-carrier protein] + L-alanine = 8-amino-7-oxononanoate + CO2 + holo-[acyl-carrier protein] | formation of an external intermediate beta-ketoacid pyridoxal-5'-phosphate aldimine, preference for L-alanine | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-carboxyhexanoyl-CoA + D-alanine | the L-isomer of the amino acid is preferred, D-alanine shows 5fold slower reaction, interaction analysis, overview | Mycobacterium tuberculosis | 8-amino-7-oxononanoate + CoA + CO2 | i.e. D-KAPA | ? | |
6-carboxyhexanoyl-CoA + L-alanine | first committed step in the biotin biosynthesis pathway | Mycobacterium tuberculosis | 8-amino-7-oxononanoate + CoA + CO2 | - |
? | |
6-carboxyhexanoyl-CoA + L-alanine | the L-isomer of the amino acid is preferred giving a 5fold faster reaction, interaction analysis, overview | Mycobacterium tuberculosis | 8-amino-7-oxononanoate + CoA + CO2 | i.e. L-KAPA | ? | |
additional information | pimeloyl-CoA forms a quinonoid with L- or D-KAPA | Mycobacterium tuberculosis | ? | - |
? | |
pimeloyl-CoA + D-alanine | - |
Mycobacterium tuberculosis | ? | - |
? | |
pimeloyl-CoA + L-alanine | - |
Mycobacterium tuberculosis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 42000, recombinant His-tagged enzyme, SDS-PAGE, x * 42249, recombinant His-tagged enzyme, mass spectrometry, x * 42194, amino acid sequence calculation | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
7-keto-8-aminopelargonic acid synthase | - |
Mycobacterium tuberculosis |
KAPA synthase | - |
Mycobacterium tuberculosis |
More | the enzyme belongs to subclass II of the aminotransferase family | Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0036 | - |
D-alanine | pH 7.7, 25°C, recombinant enzyme, with pimeloyl-CoA | Mycobacterium tuberculosis | |
0.004 | - |
pimeloyl-CoA | pH 7.7, 25°C, recombinant enzyme | Mycobacterium tuberculosis | |
0.007 | - |
L-alanine | pH 7.7, 25°C, recombinant enzyme, with pimeloyl-CoA | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.7 | - |
assay at | Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | dependent on | Mycobacterium tuberculosis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.115 | - |
D-KAPA | pH 7.7, 25°C, recombinant enzyme | Mycobacterium tuberculosis |