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Literature summary for 2.3.1.47 extracted from
- Broad substrate stereospecificity of the Mycobacterium tuberculosis 7-keto-8-aminopelargonic acid synthase: spectroscopic and kinetic studies (2006), J. Biol. Chem., 281, 25076-25088.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene Rv1569, overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Mycobacterium tuberculosis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-KAPA | product inhibition | Mycobacterium tuberculosis |  |
| additional information | no inhibition by D-alanine | Mycobacterium tuberculosis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | stopped-flow kinetics of enzyme interaction with L-alanine and D-alanine, steady-state kinetics and thermodynamics | Mycobacterium tuberculosis | |
| 0.0015 | - |
pimeloyl-CoA | pH 7.7, 25°C, recombinant enzyme | Mycobacterium tuberculosis | |
| 0.2015 | - |
D-alanine | pH 7.7, 25°C, recombinant enzyme, with pimeloyl-CoA | Mycobacterium tuberculosis | |
| 0.4675 | - |
L-alanine | pH 7.7, 25°C, recombinant enzyme, with pimeloyl-CoA | Mycobacterium tuberculosis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42000 | - |
x * 42000, recombinant His-tagged enzyme, SDS-PAGE, x * 42249, recombinant His-tagged enzyme, mass spectrometry, x * 42194, amino acid sequence calculation | Mycobacterium tuberculosis |
| 42194 | - |
x * 42000, recombinant His-tagged enzyme, SDS-PAGE, x * 42249, recombinant His-tagged enzyme, mass spectrometry, x * 42194, amino acid sequence calculation | Mycobacterium tuberculosis |
| 42249 | - |
x * 42000, recombinant His-tagged enzyme, SDS-PAGE, x * 42249, recombinant His-tagged enzyme, mass spectrometry, x * 42194, amino acid sequence calculation | Mycobacterium tuberculosis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 6-carboxyhexanoyl-CoA + L-alanine | Mycobacterium tuberculosis | first committed step in the biotin biosynthesis pathway | 8-amino-7-oxononanoate + CoA + CO2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | - |
gene Rv1569 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affiinity chromatography and gel filtration to homogeneity | Mycobacterium tuberculosis |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| pimeloyl-[acyl-carrier protein] + L-alanine = 8-amino-7-oxononanoate + CO2 + holo-[acyl-carrier protein] | formation of an external intermediate beta-ketoacid pyridoxal-5'-phosphate aldimine, preference for L-alanine | Mycobacterium tuberculosis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 6-carboxyhexanoyl-CoA + D-alanine | the L-isomer of the amino acid is preferred, D-alanine shows 5fold slower reaction, interaction analysis, overview | Mycobacterium tuberculosis | 8-amino-7-oxononanoate + CoA + CO2 | i.e. D-KAPA | ? | |
| 6-carboxyhexanoyl-CoA + L-alanine | first committed step in the biotin biosynthesis pathway | Mycobacterium tuberculosis | 8-amino-7-oxononanoate + CoA + CO2 | - |
? | |
| 6-carboxyhexanoyl-CoA + L-alanine | the L-isomer of the amino acid is preferred giving a 5fold faster reaction, interaction analysis, overview | Mycobacterium tuberculosis | 8-amino-7-oxononanoate + CoA + CO2 | i.e. L-KAPA | ? | |
| additional information | pimeloyl-CoA forms a quinonoid with L- or D-KAPA | Mycobacterium tuberculosis | ? | - |
? | |
| pimeloyl-CoA + D-alanine | - |
Mycobacterium tuberculosis | ? | - |
? | |
| pimeloyl-CoA + L-alanine | - |
Mycobacterium tuberculosis | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 42000, recombinant His-tagged enzyme, SDS-PAGE, x * 42249, recombinant His-tagged enzyme, mass spectrometry, x * 42194, amino acid sequence calculation | Mycobacterium tuberculosis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 7-keto-8-aminopelargonic acid synthase | - |
Mycobacterium tuberculosis |
| KAPA synthase | - |
Mycobacterium tuberculosis |
| More | the enzyme belongs to subclass II of the aminotransferase family | Mycobacterium tuberculosis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Mycobacterium tuberculosis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0036 | - |
D-alanine | pH 7.7, 25°C, recombinant enzyme, with pimeloyl-CoA | Mycobacterium tuberculosis | |
| 0.004 | - |
pimeloyl-CoA | pH 7.7, 25°C, recombinant enzyme | Mycobacterium tuberculosis | |
| 0.007 | - |
L-alanine | pH 7.7, 25°C, recombinant enzyme, with pimeloyl-CoA | Mycobacterium tuberculosis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.7 | - |
assay at | Mycobacterium tuberculosis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | dependent on | Mycobacterium tuberculosis | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.115 | - |
D-KAPA | pH 7.7, 25°C, recombinant enzyme | Mycobacterium tuberculosis | |
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